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Titolo:
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function
Autore:
Das, S; Varalakshmi, C; Kumari, AL; Patel, M; Khar, A;
Indirizzi:
Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India Ctr Cellular & Mol Biol Hyderabad Andhra Pradesh India 500007 desh, India
Titolo Testata:
CANCER IMMUNOLOGY IMMUNOTHERAPY
fascicolo: 8, volume: 50, anno: 2001,
pagine: 428 - 436
SICI:
0340-7004(200110)50:8<428:TCIAON>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; IFN-GAMMA PRODUCTION; DNA FRAGMENTATION; INTERFERON-GAMMA; LYMPHOCYTE-T; TUMOR-CELLS; RECEPTOR; EXPRESSION; INDUCTION; MHC;
Keywords:
tumour cells; NK cell activation; cytotoxic function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Khar, A Ctr Cellular & Mol Biol, Uppal Rd, Hyderabad 500007, Andhra Pradesh, India Ctr Cellular & Mol Biol Uppal Rd Hyderabad Andhra Pradesh India 500007
Citazione:
S. Das et al., "Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function", CANCER IMMU, 50(8), 2001, pp. 428-436

Abstract

This study examines the effect of fixed AK-5 tumour cells on rat NK cells. Co-culture of NK cells with fixed tumour cells augmented the cytotoxicity of NK cells against NK-sensitive targets, YAC-1 and AK5, and induced the secretion of IFN-gamma by NK cells. Antibody against IFN-gamma suppressed theanti-tumour activity of NK cells, whereas the addition of T cells during co-culture enhanced this activity. However, macrophages and B cells had no significant effect when present during co-culture with NK cells. All the inducible cytotoxicity was contained within the NK (CD 161(+)) and NKT (CD3(+), CD161(+)) subsets of lymphocytes. However, in the presence of T cells, the cytolytic potential of NKT cells was higher than that of NK cells alone. The augmentation of cytotoxic activity of NK cells by AK-5 cells in presence of T cells was dependent on IL-2 and IFN-gamma secretion. NK cell activation was blocked by specific antibodies to IL-2 and IFN-gamma in the presence of T cells. Interaction between fixed AK-5 cells with NK and T cell populations induced the expression of Fas-L and perforin in NK cells. These datademonstrate that fixed AK-5 cells initiated cytokine synthesis by NK cells, and the enhanced cytotoxic activity in the presence of T cells was induced as a consequence of the products secreted by activated T lymphocytes. Thepresent observations reflect the possible interactions taking place in vivo after the transplantation of AK-5 tumour in animals. They also suggest direct activation of NK cells after their interaction with the tumour cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 06:57:54