Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Homotypic cell-cell contacts stimulate the motile activity of rat prostatecancer cells
Autore:
Madeja, Z; Miekus, K; Sroka, J; Djamgoz, MBA; Korohoda, W;
Indirizzi:
Jagiellonian Univ, Jan Zurzycki Inst Mol Biol, Dept Cell Biol, PL-31120 Krakow, Poland Jagiellonian Univ Krakow Poland PL-31120 l Biol, PL-31120 Krakow, Poland Univ London Imperial Coll Sci Technol & Med, Dept Biol, London, England Univ London Imperial Coll Sci Technol & Med London England don, England
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 7, volume: 88, anno: 2001,
pagine: 776 - 786
SICI:
1464-4096(200111)88:7<776:HCCSTM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SARCOMA-CELLS; AMEBA-PROTEUS; LOCOMOTION; FIBROBLASTS; LINES; MALIGNANCY; CHEMOTAXIS; SUBSTRATA; RESPONSES; BEHAVIOR;
Keywords:
cell movement; metastasis; contact-inhibition; contact-activation; conditioned medium; autocrine motility factor; ion channel; prostate cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Madeja, Z Jagiellonian Univ, Jan Zurzycki Inst Mol Biol, Dept Cell Biol, Al Mickiewieza 3, PL-31120 Krakow, Poland Jagiellonian Univ Al Mickiewieza 3Krakow Poland PL-31120 oland
Citazione:
Z. Madeja et al., "Homotypic cell-cell contacts stimulate the motile activity of rat prostatecancer cells", BJU INT, 88(7), 2001, pp. 776-786

Abstract

Objective To characterize the effect of homotypic cell-to-cell collisions upon the motile activities of two rat prostatic cancer cell lines of markedly different metastatic potential. Materials and methods The movements of strongly and weakly metastatic MAT-LyLu and AT-2 cells. respectively, were recorded under an inverted microscope at 37 degreesC. The motile activities of the cells at various cell densities were characterized quantitatively by computer-aided tracking methods and image analysis. The following variables were assessed: speed of movement. final displacement. coefficient of movement efficiency. diffusion constant and positive flow. Results MAT-LyLu and AT-2 cells showed only limited motile activity in sparse cultures where there was little contact amongst the cells. However. under these and all other subsequent conditions tested. the motile activity ofthe MAT-LyLu cells was higher than the AT-2 cells. As the density of the cultured cells was increased (leading to more cell-to-cell contacts) there was a significant increase in motility. This effect was more pronounced for the AT-2 than for the MAT-LyLu cells. resulting in visible acceleration of movement by direct physical contact among the colliding cells. The motile activities of the tumour cells was only slightly affected by conditioned media. Conclusion Homotypic collisions between migrating prostatic cancer cells can strongly stimulate their motility. The effect of increased contact is greater on the weakly metastatic cells. such that at high cell density, the difference in the motilities of weak and strong metastatic cells is greatly reduced.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 00:49:33