Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Analysis of N-linked oligosaccharides: progress towards the characterisation of glycoprotein-linked carbohydrates
Autore:
Charlwood, J; Bryant, D; Skehel, JM; Camilleri, P;
Indirizzi:
SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut Harlow Essex England CM19 5AW ssex, England
Titolo Testata:
BIOMOLECULAR ENGINEERING
fascicolo: 5, volume: 18, anno: 2001,
pagine: 229 - 240
SICI:
1389-0344(200111)18:5<229:AONOPT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; POLYACRYLAMIDE-GEL ELECTROPHORESIS; MASS-SPECTROMETRIC IDENTIFICATION; COLONY-STIMULATING FACTOR; 2-DIMENSIONAL ELECTROPHORESIS; POSTTRANSLATIONAL MODIFICATIONS; PROTEINS; 2-AMINOACRIDONE; SCALE; MIXTURES;
Keywords:
proteomics; glycoprotein; carbohydrate; 3-(acetylamino)-6-aminoacridine; matrix-assisted laser desorption ionization time of flight mass spectrometry; two-dimensional SDS-polyacrylamide gel electrophoresis; peptide N-glycanase F;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Camilleri, P SmithKline Beecham Pharmaceut, New Frontiers Sci Pk N,3rd Ave, Harlow CM195AW, Essex, England SmithKline Beecham Pharmaceut New Frontiers Sci Pk N,3rd Ave Harlow Essex England CM19 5AW
Citazione:
J. Charlwood et al., "Analysis of N-linked oligosaccharides: progress towards the characterisation of glycoprotein-linked carbohydrates", BIOMOL ENG, 18(5), 2001, pp. 229-240

Abstract

The covalent attachment of carbohydrate to proteins is a very common co- or post-translational event in the biosynthesis of glycoproteins. The type and heterogeneity of these oligosaccharides can affect a range of physico-chemical and biological properties of a glycoprotein. Thus the development ofsensitive, reliable and robust analytical methods for carbohydrate analysis is important in the pharmaceutical industry, especially in the recombinant production of experimental and therapeutic glycoproteins. In this report we have reviewed methodology for the in-gel enzymatic release of N-linked oligosaccharides from glycoproteins separated by electrophoresis. These oligosaccharides are derivatised by reductive amination using 3-acetamido-6-aminoacridine (AA-Ac), a novel, highly fluorescent probe. A major advantage ofthis technique is that glycan derivatives are amenable to analysis by an array of chromatographic and mass spectrometric methods, allowing the resolution and characterisation of a wide variety of glycan structures. It is hoped that in due course the methodology described will be applied to proteomics studies, especially in identifying the role of carbohydrate in protein function and disease. (C) 2001 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 10:39:58