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Titolo:
Interrelationships among biological activity, disulfide bonds, secondary structure, and metal ion binding for a chemically synthesized 34-amino-acid peptide derived from alpha-fetoprotein
Autore:
MacColl, R; Eisele, LE; Stack, RF; Hauer, C; Vakharia, DD; Benno, A; Kelly, WC; Mizejewski, GJ;
Indirizzi:
New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 & Res, Albany, NY 12201 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 2-3, volume: 1528, anno: 2001,
pagine: 127 - 134
SICI:
0304-4165(20011003)1528:2-3<127:IABADB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZINC FINGER PEPTIDES; BREAST-CANCER; PROTEIN; COBALT(II); DOMAIN; ESTRADIOL; TRIFLUOROETHANOL; COORDINATION; RUBREDOXIN; FRAGMENTS;
Keywords:
alpha-fetoprotein; anti-growth and anti-cancer peptide; Zn(II)-peptide complex; Co(II) spectroscopic probe for peptide binding; peptide conformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: MacColl, R New York State Dept Hlth, Wadsworth Ctr Labs & Res, Pob 509, Albany, NY 12201 USA New York State Dept Hlth Pob 509 Albany NY USA 12201 12201 USA
Citazione:
R. MacColl et al., "Interrelationships among biological activity, disulfide bonds, secondary structure, and metal ion binding for a chemically synthesized 34-amino-acid peptide derived from alpha-fetoprotein", BBA-GEN SUB, 1528(2-3), 2001, pp. 127-134

Abstract

A 34-amino-acid peptide has been chemically synthesized based on a sequence from human ct-fetoprotein. The purified peptide is active in anti-growth assays when freshly prepared in pH 7.4 buffer at 0.20 g/l, but this peptideslowly becomes inactive. This functional change is proven by mass spectrometry to be triggered by the formation of an intrapeptide disulfide bond between the two cysteine residues on the peptide. Interpeptide cross-linking does not occur. The active and inactive forms of the peptide have almost identical secondary structures as shown by circular dichroism (CD). Zinc ions bind to the active peptide and completely prevents formation of the inactive form. Cobalt(II) ions also bind to the peptide, and the UV-Vis absorptionspectrum of the cobalt-peptide complex shows that: (1) a near-UV sulfur-to-metal-ion charge-transfer band had a molar extinction coefficient consistent with two thiolate bonds to Co(II); (2) the lowest-energy visible d-d transition maximum at 659 nm, also, demonstrated that the two cysteine residues are ligands for the metal ion; (3) the d-d molar extinction coefficient showed that the metal ion-ligand complex was in a distorted tetrahedral symmetry. The peptide has two cysteines, and it is speculated that the other two metal ion ligands might be the two histidines. The Zn(II)- and Co(II)-peptide complexes had similar peptide conformations as indicated by their ultraviolet CID spectra, which differed very slightly from that of the free peptide. Surprisingly, the cobalt ions acted in the reverse of the zinc ions in that, instead of stabilizing anti-growth form of the peptide, they catalyzed its loss. Metal ion control of peptide function is a saliently interesting concept. Calcium ions, in the conditions studied, apparently do not bind to the peptide. Trifluoroethanol and temperature (60 degreesC) affected the secondary structure of the peptide, and the peptide was found capable ofassuming various conformations in solution. This conformational flexibility may possibly be related to the biological activity of the peptide. (C) 2001 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:40:52