Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Platelet damage caused by the centrifugal pump: Laser-light scattering analysis of aggregation patterns
Autore:
Tanaka, M; Kawahito, K; Adachi, H; Isawa, T; Ino, T;
Indirizzi:
Jichi Med Sch, Omiya Med Ctr, Dept Cardiovasc Surg, Omiya, Saitama 3300834, Japan Jichi Med Sch Omiya Saitama Japan 3300834 , Omiya, Saitama 3300834, Japan Jichi Med Sch, Omiya Med Ctr, Dept Neurosurg, Omiya, Saitama, Japan Jichi Med Sch Omiya Saitama Japan Dept Neurosurg, Omiya, Saitama, Japan
Titolo Testata:
ARTIFICIAL ORGANS
fascicolo: 9, volume: 25, anno: 2001,
pagine: 719 - 723
SICI:
0160-564X(200109)25:9<719:PDCBTC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIOPULMONARY BYPASS OPERATIONS; VENTRICULAR ASSIST; BLOOD-PLATELETS; IN-VITRO; ACTIVATION;
Keywords:
centrifugal pump; laser-light scattering method; platelet aggregation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, M Jichi Med Sch, Omiya Med Ctr, Dept Cardiovasc Surg, 1-847 Amanuma, Omiya, Saitama 3300834, Japan Jichi Med Sch 1-847 Amanuma Omiya Saitama Japan 3300834 4, Japan
Citazione:
M. Tanaka et al., "Platelet damage caused by the centrifugal pump: Laser-light scattering analysis of aggregation patterns", ARTIF ORGAN, 25(9), 2001, pp. 719-723

Abstract

Platelets are damaged by shear force during centrifugal pumping; however, the mechanism of this damage has not been fully investigated. A new laser-light scattering (LS) assay method enables quantification of realtime chan-es in the number of platelet aggregates of different sizes. Using this method, we assessed the kinetics of platelet damage caused by the centrifugal pump from the standpoint of platelet aggregation capacity. Conventional optical density (OD) and LS methods were used to measure platelet aggregation with a Kowa AG-10 aggregometer. Platelet aggregation in fresh human blood wasevaluated in a mock circuit for 3 h under a flow rate of 5.0 L/min and a pressure head of 100 mm Hg. Test samples were obtained before pumping for control, and at 1, 2, and 3 h after the start of pumping. The test series wasbegun 8 times. Aggregation after stimulation by 2.0 mug/ml collagen was determined; small (9 to 25 mum), medium (25 to 50 mum), and large (50 to 70 mum) aggregates were counted by the LS method. OD measurement at hourly intervals showed significant reduction in platelet aggregation. The LS method showed that generation of small and medium aggregates was not suppressed during pumping, but that generation of large aggregates was significantly reduced at 2 and 3 h of pumping. Platelet aggregation is significantly suppressed during centrifugal pumping, and the resulting platelet dysfunction is due mainly to inhibited development of small aggregates into larger aggregates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 00:28:42