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Titolo:
High beryllium-stimulated TNF-alpha is associated with the-308 TNF-alpha promoter polymorphism and with clinical severity in chronic beryllium disease
Autore:
Maier, LA; Sawyer, RT; Bauer, RA; Kittle, LA; Lympany, P; McGrath, D; Dubois, R; Daniloff, E; Rose, CS; Newman, LS;
Indirizzi:
Natl Jewish Med & Res Ctr, Dept Med, Div Environm & Occupat Hlth Sci, Denver, CO 80206 USA Natl Jewish Med & Res Ctr Denver CO USA 80206 h Sci, Denver, CO 80206 USA Univ Colorado, Hlth Sci Ctr, Dept Med, Div Pulm Sci & Crit Care Med, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 i & Crit Care Med, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Prevent Med & Biometr, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 ent Med & Biometr, Denver, CO 80262 USA Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, Dept Occupat & Environm Med, Interstitial Lung Dis Unit, London, England Univ London Imperial Coll Sci Technol & Med London England don, England
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
fascicolo: 7, volume: 164, anno: 2001,
pagine: 1192 - 1199
SICI:
1073-449X(20011001)164:7<1192:HBTIAW>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; SYSTEMIC LUPUS-ERYTHEMATOSUS; SEQUENCE-SPECIFIC PRIMERS; ALVEOLAR MACROPHAGES; PULMONARY SARCOIDOSIS; IN-VITRO; RELEASE; GENE; INTERLEUKIN-6; RELEVANCE;
Keywords:
chronic beryllium disease; TNF-alpha; genetic polymorphisms; functional genetics; genetic susceptibility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Maier, LA Natl Jewish Med & Res Ctr, Dept Med, Div Environm & Occupat HlthSci, 1400Jackson St,Room M210, Denver, CO 80206 USA Natl Jewish Med & Res Ctr 1400 Jackson St,Room M210 Denver CO USA 80206
Citazione:
L.A. Maier et al., "High beryllium-stimulated TNF-alpha is associated with the-308 TNF-alpha promoter polymorphism and with clinical severity in chronic beryllium disease", AM J R CRIT, 164(7), 2001, pp. 1192-1199

Abstract

Beryllium (Be)-antigen stimulates tumor necrosis factor-alpha (TNF-alpha) from bronchoalveolar lavage (BAL) cells in chronic beryllium disease (CBD). This study tested the hypothesis that high concentrations of Be-stimulatedTNF-alpha are related to polymorphisms in the TNF-alpha promoter and clinical markers of disease severity in CBD. Demographic and clinical information was obtained from patients with CBD (n = 20). TNF-alpha concentrations were measured in BAL cell culture supernatant by ELISA. A prior), we categorized CBD subjects as either high or low TNF-alpha producers using a cutoff of 1,500 pg/ml. The TNF-alpha promoter sequence, +64 to -1045, was determined by direct sequencing. Human leukocyte-associated antigen (HLA)-DPB1 and -DRB1 genotyping was determined by polymerase chain reaction (PCR). High Be-stimulated TNF-alpha was associated with TNF2 alleles, Hispanic ethnicity, presence of HLA-DPB1 Glu69, and absence of HLA-DR4. Be-stimulated TNF-alphaconcentrations correlated with markers of disease severity, including chest radiograph, beryllium lymphocyte proliferation, and spirometry. We found no novel TNF-alpha promoter polymorphisms. These data suggest that the TNF2A allele at -308 in the TNF-alpha promoter region is a functional polymorphism, associated with a high level of Be-antigen-stimulated TNF-alpha and that these high TNF-alpha levels indicate disease severity in CBD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:41:59