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Titolo:
Enhancement of in vitro hsp72 expression by placental IL-6
Autore:
Miranda, S; Gentile, T; Margni, R;
Indirizzi:
Univ Buenos Aires, Fac Farm & Bioquim, CONICET, Inst Estudios Inmunidad Humoral, RA-1113 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1113 Aires, DF, Argentina
Titolo Testata:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 5, volume: 46, anno: 2001,
pagine: 358 - 365
SICI:
1046-7408(200111)46:5<358:EOIVHE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOCK PROTEIN EXPRESSION; ASYMMETRIC ANTIBODIES; IN-VITRO; MOLECULAR CHAPERONES; MATING COMBINATION; HUMAN TROPHOBLAST; CELLULAR CLONE; MESSENGER-RNA; INTERLEUKIN-6; CYTOKINES;
Keywords:
glycosylation; hsp70; hybridoma; IL-6; mouse placenta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Miranda, S Univ Buenos Aires, Fac Farm & Bioquim, CONICET, Inst Estudios Inmunidad Humoral, Junin 956 4P, RA-1113 Buenos Aires, DF, Argentina Univ Buenos Aires Junin 956 4P Buenos Aires DF Argentina RA-1113
Citazione:
S. Miranda et al., "Enhancement of in vitro hsp72 expression by placental IL-6", AM J REPROD, 46(5), 2001, pp. 358-365

Abstract

PROBLEM: To give an approach in order to elucidate the mechanism by which placental IL-6 induce modifications in the glycosylation status of immunoglobulins, in the present work, we investigate a putative relationship between a stimulus by placental IL-6 and expression of cytoplasmic "hsp70 family proteins" in an in vitro model. METHODS OF STUDY: Supernatants of cultures of placentae obtained from primiparous and multiparous AKR/J x AKR/J and AKR/J x BALB/c mouse crossbreedings were added to mouse IgG1 hybridoma cultures which produced symmetric andasymmetric anti-dinitrophenol (anti-DNP) antibodies. Analyses of the expression of inducible hsp72/constitutive hsp73 in cellular lysates obtained from hybridomas cultured, in the presence of rmIL-6 or crude murine placentalculture supernatants; followed by neutralization assays with anti IL-6, were performed. In addition, the level of IL-6 present in the employed placental culture supernatants was determined and compared with the placental hsp70-inducing effect. RESULTS: These experiments showed that mouse placentae were able to release IL-6 in vitro. In addition, mouse placental supernatants (PS) containing over 1,000 pg/mL of IL-6 enhanced the expression of the inducible isoform hsp72 in the employed hybridomas. This effect was abolished when the hsp70-inducing PS were previously incubated with anti-mIL6 antibody. CONCLUSIONS: These observations indicate that mouse placentae produce different titers of IL-6 and suggest that IL-6 appears to be the unique mouse placental factor able to induce in vitro hsp72 synthesis. A relationship with the increased synthesis of anti-paternal antigen asymmetric antibodies, previously observed during pregnancy, is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:34:47