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Titolo:
Polyunsaturated fatty acids stimulate hepatic UCP-2 expression via a PPAR alpha-mediated pathway
Autore:
Armstrong, MB; Towle, HC;
Indirizzi:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USAUniv Minnesota Minneapolis MN USA 55455 iophys, Minneapolis, MN 55455 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 6, volume: 281, anno: 2001,
pagine: E1197 - E1204
SICI:
0193-1849(200112)281:6<E1197:PFASHU>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNCOUPLING PROTEIN-2 EXPRESSION; BROWN ADIPOSE-TISSUE; GENE-EXPRESSION; CULTURED-HEPATOCYTES; RECEPTOR-GAMMA; UP-REGULATION; TRANSCRIPTION; OBESITY; LIVER; MICE;
Keywords:
uncoupling protein; prostaglandins; energy metabolism; peroxisome proliferator-activated receptor-alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Towle, HC 6-155 Jackson Hall,321 Church St SE, Minneapolis, MN 55455 USA 6-155 Jackson Hall,321 Church St SE Minneapolis MN USA 55455 SA
Citazione:
M.B. Armstrong e H.C. Towle, "Polyunsaturated fatty acids stimulate hepatic UCP-2 expression via a PPAR alpha-mediated pathway", AM J P-ENDO, 281(6), 2001, pp. E1197-E1204

Abstract

The discovery of homologs of the brown fat uncoupling protein(s) (UCP) UCP-2 and UCP-3 revived the hypothesis of uncoupling protein involvement in the regulation of energy metabolism. Thus we hypothesized that UCP-2 would beregulated in the hepatocyte by fatty acids, which are known to control other energy-related metabolic processes. Treatment with 250 muM palmitic acidwas without effect on UCP-2 expression, whereas 250 muM oleic acid exhibited a modest eightfold increase. Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, exerted a 50-fold upregulation of UCP-2 that was concentration dependent. This effect was seen within 12 h and was maximal by 36 h. Aspirin blocked the induction of UCP-2 by EPA, indicating involvement of the prostaglandin pathway. Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitableincrease in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. The peroxisome proliferator-activated receptor-alpha (PPAR alpha) agonist Wy-14643 stimulated UCP-2 mRNA levels as effectively as EPA. These data indicate that UCP-2 is upregulated by polyunsaturated fatty acids, potentially through a prostaglandin/PPAR alpha -mediated pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:45:57