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Titolo:
Role of JNK in hypertonic activation of Cl--dependent Na+/H+ exchange in Xenopus oocytes
Autore:
Goss, GG; Jiang, LW; Vandorpe, DH; Kieller, D; Chernova, MN; Robertson, M; Alper, SL;
Indirizzi:
Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada Univ Alberta Edmonton AB Canada T6G 2E9 Sci, Edmonton, AB T6G 2E9, Canada Beth Israel Deaconess Med Ctr, Mol Med Unit, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 t, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr, Renal Unit, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 t, Boston, MA 02215 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA Harvard Univ BostonMA USA 02215 Sch Med, Dept Med, Boston, MA 02215 USA Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02215 USA Harvard Univ Boston MA USA 02215 ed, Dept Cell Biol, Boston, MA 02215 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 281, anno: 2001,
pagine: C1978 - C1990
SICI:
0363-6143(200112)281:6<C1978:ROJIHA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA-H EXCHANGE; SHRINKAGE-INDUCED ACTIVATION; BARNACLE MUSCLE-FIBERS; PROTEIN-KINASE-C; LAEVIS OOCYTES; SIGNAL-TRANSDUCTION; CYCLOOXYGENASE-2 EXPRESSION; FUNCTIONAL-CHARACTERIZATION; TRANSPORT PATHWAYS; INTRACELLULAR PH;
Keywords:
Na+/H+ exchange; c-Jun NH2-terminal kinase; extracellular signal-regulated kinase; p38; transport; SP600125; U-0126;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Goss, GG Univ Alberta, Dept Biol Sci, Rm Z-512,Biol Sci Bldg, Edmonton, ABT6G 2E9,Canada Univ Alberta Rm Z-512,Biol Sci Bldg Edmonton AB Canada T6G 2E9 da
Citazione:
G.G. Goss et al., "Role of JNK in hypertonic activation of Cl--dependent Na+/H+ exchange in Xenopus oocytes", AM J P-CELL, 281(6), 2001, pp. C1978-C1990

Abstract

In the course of studying the hypertonicity-activated ion transporters in Xenopus oocytes, we found that activation of endogenous oocyte Na+/H+ exchange activity (xoNHE) by hypertonic shrinkage required Cl-, with an EC50 forbath [Cl-] of similar to3 mM. This requirement for chloride was not supported by several nonhalide anions and was not shared by xoNHE activated by acid loading. Hypertonicity-activated xoNHE exhibited an unusual rank order of inhibitory potency among amiloride derivatives and was blocked by Cl- transport inhibitors. Chelation of intracellular Ca2+ by injection of EGTA blocked hypertonic activation of xoNHE, although many inhibitors of Ca2+-related signaling pathways were without inhibitory effect. Hypertonicity activated oocyte extracellular signal-regulated kinase 1/2 (ERK1/2), but inhibitors of neither ERK1/2 nor p38 prevented hypertonic activation of xoNHE. However, hypertonicity also stimulated a Cl- dependent increase in c-Jun NH2-terminal kinase (JNK) activity. Inhibition of JNK activity prevented hypertonic activation of xoNHE but not activation by acid loading. We conclude that hypertonic activation of Na+/H+ exchange in Xenopus oocytes requires Cl- and is mediated by activation of JNK.

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Documento generato il 20/01/20 alle ore 08:14:00