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Titolo:
Developmental changes in ryanodine- and IP3-sensitive Ca2+ pools in ovine basilar artery
Autore:
Nauli, SM; Williams, JM; Akopov, SE; Zhang, L; Pearce, WJ;
Indirizzi:
Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Dept Physiol, Loma Linda, CA92350 USA Loma Linda Univ Loma Linda CA USA 92350 Physiol, Loma Linda, CA92350 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 281, anno: 2001,
pagine: C1785 - C1796
SICI:
0363-6143(200112)281:6<C1785:DCIRAI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; SARCOPLASMIC-RETICULUM; CEREBRAL-ARTERIES; ENDOPLASMIC-RETICULUM; BINDING-SITES; CAFFEINE; RELEASE; STORES; MATURATION;
Keywords:
cerebral arteries; cerebrovascular circulation; furaptra; maturation; sarcoplasmic reticulum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Pearce, WJ Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Dept Physiol, Loma Linda, CA92350 USA Loma Linda Univ Loma Linda CA USA 92350 oma Linda, CA92350 USA
Citazione:
S.M. Nauli et al., "Developmental changes in ryanodine- and IP3-sensitive Ca2+ pools in ovine basilar artery", AM J P-CELL, 281(6), 2001, pp. C1785-C1796

Abstract

To explore the hypothesis that cerebrovascular maturation alters ryanodine- and inositol 1,4,5- trisphosphate (IP3)-sensitive Ca2+ pool sizes, we measured total intracellular Ca2+ with Ca-45 and the fractions of intracellular Ca2+ released by IP3 and/or caffeine in furaptra-loaded permeabilized basilar arteries from nonpregnant adult and term fetal (139-141 days) sheep. Ca2+ mass (nmol/mg dry weight) was similar in adult (1.60 +/-0.18) and fetal(1.71 +/-0.16) arteries in the pool sensitive to IP3 alone but was significantly lower for adult (0.11 +/-0.01) than for fetal (1.22 +/-0.11) arteries in the pool sensitive to ryanodine alone. The pool sensitive to both ryanodine and IP3 was also smaller in adult (0.14 +/-0.01) than in fetal (0.85 /-0.08) arteries. Because the Ca2+ fraction in the ryanodine- IP3 pool wassmall in both adult (5 +/-1%) and fetal (7 +/-4%) arteries, the IP3 and ryanodine pools appear to be separate in these arteries. However, the pool sensitive to neither IP3 nor ryanodine was 10-fold smaller in adult (0.87 +/-0.10) than in fetal (8.78 +/-0.81) arteries, where it accounted for 72% of total intracellular membrane-bound Ca2+. Thus, during basilar artery maturation, intracellular Ca2+ mass plummets in noncontractile pools, decreases modestly in ryanodine- sensitive pools, and remains constant in IP3-sensitive pools. In addition, age-related increases in IP3 efficacy must involve factors other than IP3 pool size alone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:51:09