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Titolo:
RHD sequencing: a new tool for decision making on transfusion therapy and provision of Rh prophylaxis
Autore:
Legler, TJ; Maas, JH; Kohler, M; Wagner, T; Daniels, GL; Perco, P; Panzer, S;
Indirizzi:
Univ Gottingen, Dept Transfus Med, D-37075 Gottingen, Germany Univ Gottingen Gottingen Germany D-37075 Med, D-37075 Gottingen, Germany Univ Clin Graz, Dept Blood Grp Serol & Transfus Med, Graz, Austria Univ Clin Graz Graz Austria ood Grp Serol & Transfus Med, Graz, Austria Bristol Inst Transfus Sci, Bristol, Avon, England Bristol Inst Transfus Sci Bristol Avon England i, Bristol, Avon, England Univ Vienna, Dept Blood Grp Serol, Vienna, Austria Univ Vienna Vienna Austria ienna, Dept Blood Grp Serol, Vienna, Austria
Titolo Testata:
TRANSFUSION MEDICINE
fascicolo: 5, volume: 11, anno: 2001,
pagine: 383 - 388
SICI:
0958-7578(200110)11:5<383:RSANTF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
WEAK-D-PHENOTYPES;
Keywords:
blood group determination; molecular biology; Rh prophylaxis; RHD; sequencing; transfusion policy; weak D;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Legler, TJ Univ Gottingen, Dept Transfus Med, Robert Koch Str 40, D-37075 Gottingen, Germany Univ Gottingen Robert Koch Str 40 Gottingen Germany D-37075 ny
Citazione:
T.J. Legler et al., "RHD sequencing: a new tool for decision making on transfusion therapy and provision of Rh prophylaxis", TRANSFUS M, 11(5), 2001, pp. 383-388

Abstract

The serological differentiation of weak D from partial D, D-negative and D-positive is not always unequivocal. Therefore, sequencing of the RHD gene is required in some cases. Very recently, several new differences between RHD and RHCE have been identified which permitted us to design primers closeto the exon/intron boundaries of the RHD-exons. We evaluated these primersin 83 D-positive and 18 D-negative blood donors and applied the new methodfor the characterization of the RHD gene in six individuals with weak D phenotype. The amplification reactions were concordant with serological findings in 100 of 101 donors (99.0%). In one D-positive donor the PCR for exons2 and 5 gave a negative result, while the sequence of the remaining eight exons was unchanged. By sequencing samples with very weak D serological reactions, we identified weak D type 4.2.2 and weak D type 15, both previouslyreported to be associated with anti-D-alloimmunization. Consequently, we recommended the selection of D-negative blood in the weak D type 4.2.2 patient, and the provision of Rh prophylaxis for pregnant women with weak D type15. In summary, a new RHD sequencing method was developed which can be applied if serological reactions are inconclusive.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:17:57