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Titolo:
Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells
Autore:
Khandekar, SS; Silverman, C; Wells-Marani, J; Bacon, AM; Birrell, H; Brigham-Burke, M; DeMarini, DJ; Jonak, ZL; Camilleri, P; Fishman-Lobell, J;
Indirizzi:
SmithKline Beecham Pharmaceut, Dept Analyt Sci, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Oncol Res, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Prot Biochem, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Computat Analyt & Struct Sci, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Comparat Genet, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Harlow CM19 5AD, Essex, England SmithKline Beecham Pharmaceut Harlow Essex England CM19 5AD ssex, England
Titolo Testata:
PROTEIN EXPRESSION AND PURIFICATION
fascicolo: 2, volume: 23, anno: 2001,
pagine: 301 - 310
SICI:
1046-5928(200111)23:2<301:DOCSNT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; HERPESVIRUS ENTRY MEDIATOR; SITE-DIRECTED MUTAGENESIS; HUMAN INTERFERON-GAMMA; BIOLOGICAL-ACTIVITY; DENDRITIC CELLS; T-CELLS; LIGAND; RECEPTOR; GLYCOSYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Fishman-Lobell, J SmithKline Beecham Pharmaceut, Dept Analyt Sci, 709 Swedeland Rd, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut 709 Swedeland Rd King Of Prussia PA USA 19406
Citazione:
S.S. Khandekar et al., "Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells", PROT EX PUR, 23(2), 2001, pp. 301-310

Abstract

CD40-CD154 (CD40 ligand) interactions are essential for the development ofprotective immunity. Previous studies have described the CD40 binding siteas a shallow groove formed between two monomers of CD154. However, these studies have not examined the structure or biological function of the carbohydrate on CD154. Human CD154 contains a single N-linked glycosylation site at asparagine 240. We have characterized the interactions between CD40 and soluble (s) CD154 in which sCD154 contains different types of carbohydrates. Detailed carbohydrate analysis revealed high-mannose structures on sCD154purified from Pichia pastoris, whereas CD154 purified from Chinese hamsterovary EIA contained heterogeneous populations of complex carbohydrates. sCD154 purified from. either system was trimeric, it bound to CD40 with similar affinities of 10-30 nM, and it functionally induced CD69 and CD95 expression on primary B cells. Together, these results indicate that the presenceof varied types of N-linked glycans on asparagine 240 of CD154 does not play a significant role in the CD40-CD154 interactions. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:17:10