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Titolo:
Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription
Autore:
Yu, F; Zingler, N; Schumann, G; Stratling, WH;
Indirizzi:
Univ Hamburg, Hosp Eppendorf, Inst Med Biochem & Mol Biol, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 Mol Biol, D-20246 Hamburg, Germany Univ Hamburg, Heinrich Pette Inst Expt Virol & Immunol, D-20251 Hamburg, Germany Univ Hamburg Hamburg Germany D-20251 & Immunol, D-20251 Hamburg, Germany
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 21, volume: 29, anno: 2001,
pagine: 4493 - 4501
SICI:
0305-1048(20011101)29:21<4493:MP2RLE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-FREQUENCY RETROTRANSPOSITION; HISTONE DEACETYLASE COMPLEX; CHROMOSOMAL PROTEIN; L1 RETROTRANSPOSITION; CULTURED-CELLS; RETT-SYNDROME; HUMAN GENOME; MECP2; SEQUENCE; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Stratling, WH Univ Hamburg, Hosp Eppendorf, Inst Med Biochem & Mol Biol, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 Germany
Citazione:
F. Yu et al., "Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription", NUCL ACID R, 29(21), 2001, pp. 4493-4501

Abstract

In order to explore the defense mechanism by which retrotransposons are repressed, we assessed the ability of methyl-CpG-binding protein 2, MeCP2, toinfluence LINE-1 (L1) and Alu transcription and, furthermore, L1 retrotransposition. In transient transfection assays, targeting of the transcriptional-repression domain (TRD) of MeCP2 (via a linked Gal4 DNA-binding domain) to the transcriptional start site of L1 promoter-driven reporter constructsefficiently repressed transcription. The Gal4-linked TRD of the related methyl-CpG-binding protein MBD1 also repressed transcription but not that of MBD2. Furthermore, full-length MeCP2 effectively repressed transcription ofa HpaII-methylated L1 reporter. Secondly, we used a genetic assay employing a full-length neo-marked L1 reporter construct to study L1 retrotransposition. We found the Gal4-linked TRD of MeCP2 to repress effectively L1 retrotransposition when targeted to the retrotransposition reporter. Retrotransposition was also reduced in response toin vitro HpaII methylation of the reporter and was further decreased by co-expressed full-length MeCP2. In striking contrast expression of the Gal4-linked TRD of MeCP2 had no inhibiting effect on transcription of an AluSx reporter tagged with a 7S-upstream sequence. Furthermore, full-length MeCP2 abrogated the methylation-induced repression of this reporter. Our results indicate that MeCP2 serves a role in repression of L1 expression and retrotransposition but has no inhibiting effect on Alu transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:38:51