Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF
Autore:
Alsina, B; Vu, T; Cohen-Cory, S;
Indirizzi:
Univ Calif Los Angeles, Dept Psychiat & Biobehav Sci, Mental Retardat Res Ctr, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
NATURE NEUROSCIENCE
fascicolo: 11, volume: 4, anno: 2001,
pagine: 1093 - 1101
SICI:
1097-6256(200111)4:11<1093:VSFIAO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; NEUROMUSCULAR-JUNCTIONS; HIPPOCAMPAL-NEURONS; EXCITATORY SYNAPSES; NEUROTROPHIC FACTOR; DENDRITIC GROWTH; C-ELEGANS; SYNAPTOGENESIS; PROTEIN; ARBORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Cohen-Cory, S Univ Calif Los Angeles, Dept Psychiat & Biobehav Sci, MentalRetardat Res Ctr, 760 Westwood Plaza,NPI 58-258, Los Angeles, CA 90095 USAUniv Calif Los Angeles 760 Westwood Plaza,NPI 58-258 Los Angeles CA USA 90095
Citazione:
B. Alsina et al., "Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF", NAT NEUROSC, 4(11), 2001, pp. 1093-1101

Abstract

Dynamic developmental changes in axon arbor morphology may directly reflect the formation, stabilization and elimination of synapses. We used dual-color imaging to study, in the live, developing animal, the relationship between axon arborization and synapse formation at the single cell level, and to examine the participation of brain-derived neurotrophic factor (BDNF) in synaptogenesis. Green fluorescent protein (GFP)-tagged synaptobrevin II served as a marker to visualize synaptic sites in individual fluorescently labeled Xenopus optic axons. Time-lapse confocal microscopy revealed that although most synapses remain stable, synapses are also formed and eliminated as axons branch and increase their complexity. Most new branches originated at GFP-labeled synaptic sites. Increasing BDNF levels significantly increased both axon arborization and synapse number, with BDNF increasing synapse number per axon terminal. The ability to visualize central synapses in realtime provides insights about the dynamic mechanisms underlying synaptogenesis, and reveals BDNF as a modulator of synaptogenesis in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:21:54