Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The inhibition of DNA repair capacity by stilbene estrogen in Leydig cells: its implications in the induction of instability in the testicular genome
Autore:
DuMond, JW; Roy, D;
Indirizzi:
Univ Alabama, Dept Environm Hlth Sci, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 m Hlth Sci, Birmingham, AL 35294 USA
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 483, anno: 2001,
pagine: 27 - 33
SICI:
1386-1964(20011101)483:1-2<27:TIODRC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPOSURE; DIETHYLSTILBESTROL; CHEMICALS; CANCER;
Keywords:
DNA repair; host cell reactivation; Leydig cells; DES; estrogen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Roy, D Univ Alabama, Dept Environm Hlth Sci, 317 Ryals Bldg,1665 Univ Blvd, Birmingham, AL 35294 USA Univ Alabama 317 Ryals Bldg,1665 Univ Blvd Birmingham AL USA 35294
Citazione:
J.W. DuMond e D. Roy, "The inhibition of DNA repair capacity by stilbene estrogen in Leydig cells: its implications in the induction of instability in the testicular genome", MUT RES-F M, 483(1-2), 2001, pp. 27-33

Abstract

In this study, we examined the effect of stilbene estrogen, diethylstilbestrol (DES), on the DNA repair capacity of mouse Leydig cells using the hostcell reactivation assay. Cells transfected with UV-damaged plasmids, undamaged plasmids, or no plasmids (sham treatment) were grown in serum, serum-free, or DES plus serum-free medium. The ser-um-grown cells which have a shorter cell cycle time (16 h) showed a 40% decrease in DNA repair capacity when compared to serum-free cells with a longer cell cycle time (25 h). A significant decrease in the DNA repair capacity of the Leydig cells exposed toDES was observed compared to untreated cells grown in a ser-um-free environment (P < 0.05). The effect of DES on DNA repair in Leydig cells was dose dependent. We have recently shown that DES stimulates the growth of Leydig cells. Stimulatory growth of Leydig cells coupled with a decrease in DNA repair capacity by DES may allow the accumulation of mutagenic lesions in DNA. The mutagenic lesions may result from the attack of redox cycling products of DES and/or errors of replication. This, in turn, may produce alterations in the genome of Leydig cells resulting in genetically unstable cells that may serve as precursor cells for testicular carcinogenesis. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 15:23:41