Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HLA-DR expression in B-lymphocytes in vitro is not suppressed by the absence of exogenous antigens
Autore:
Park, JH; Lee, YJ; Na, SY; Cho, EW; Kim, KL;
Indirizzi:
Seoul Natl Univ, Coll Dent, Dept Oral Anat, Seoul 110749, South Korea Seoul Natl Univ Seoul South Korea 110749 Anat, Seoul 110749, South Korea Korea Res Inst Biosci & Biotechnol, Prot Engn Lab, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 2, volume: 12, anno: 2001,
pagine: 164 - 172
SICI:
1016-8478(20011031)12:2<164:HEIBIV>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-II MOLECULES; CELL-SURFACE EXPRESSION; INVARIANT CHAIN; PRESENTING CELLS; FUSION PROTEINS; MICE LACKING; T-CELL; PEPTIDES; DISSOCIATION; COMPARTMENTS;
Keywords:
antigen presentation; HLA-DR; MHC class II; serum- and protein-free medium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, KL Seoul Natl Univ, Coll Dent, Dept Oral Anat, Seoul 110749, South Korea Seoul Natl Univ Seoul South Korea 110749 oul 110749, South Korea
Citazione:
J.H. Park et al., "HLA-DR expression in B-lymphocytes in vitro is not suppressed by the absence of exogenous antigens", MOL CELLS, 12(2), 2001, pp. 164-172

Abstract

The proper loading of exogenous peptide antigens affects the transport andcell surface expression of MHC class II molecules. In the present study, the goal was to determine to what extent this step determines the cell surface expression level of MHC class II molecules, such as the HLA-DR. EBV-transformed B-cells, were cultured either in a serum- and protein-free medium, or in a medium that contained different concentrations of exogenous antigens. Using HLA-DR-specific antibodies, the induction of the MHC class II expression was observed in cells that were cultured under serum- and protein-free conditions, when compared to those cultured with exogenous protein antigens. This upregulation was completely suppressed to the normal level by theaddition of a high concentration of hen egg lysozyme to the serum- and protein-free medium. This indicates that exogenous proteins regulate the HLA-DR expression. To further examine whether this modulation is controlled at the transcription level, the expression of the HLA-DR beta -chain mRNA was analyzed by reverse transcription-PCR and Northern blots. The same levels ofHLA-DRB mRNA were detectable in both culture conditions, indicating that the present observation is dependent on some regulatory mechanisms at the post-transcriptional level. This might include a different pathway for trafficking of HLA-DR molecules to the cell surface, since peptide-binding assaysrevealed that a high proportion of cell surface HLA-DR molecules under theserum- and protein-free condition were transported to the cell surface without associated peptide antigens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 17:00:20