Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A population of PC12 cells that is initiating apoptosis can be rescued by nerve growth factor
Autore:
Francois, F; Godinho, MJ; Dragunow, M; Grimes, ML;
Indirizzi:
Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand Massey Univ Palmerston North New Zealand Palmerston North, New Zealand Univ Auckland, Dept Pharmacol & Clin Pharmacol, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 Pharmacol, Auckland 1, New Zealand
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 4, volume: 18, anno: 2001,
pagine: 347 - 362
SICI:
1044-7431(200110)18:4<347:APOPCT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPRIVED SYMPATHETIC NEURONS; ELEMENT-BINDING PROTEIN; ISCHEMIC BRAIN-DAMAGE; CYTOCHROME-C; DNA-FRAGMENTATION; ENDONUCLEASE ACTIVITY; TRANSCRIPTION FACTOR; SIGNAL-TRANSDUCTION; CASPASE ACTIVATION; FACTOR DEPRIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
93
Recensione:
Indirizzi per estratti:
Indirizzo: Grimes, ML Univ Montana, Div Biol Sci, Missoula, MT 59812 USA Univ Montana Missoula MT USA 59812 Sci, Missoula, MT 59812 USA
Citazione:
F. Francois et al., "A population of PC12 cells that is initiating apoptosis can be rescued by nerve growth factor", MOL CELL NE, 18(4), 2001, pp. 347-362

Abstract

Programmed cell death, or apoptosis, occurs asynchronously in neuronal cells. To overcome this asynchrony, rat pheochromocytoma (PC12) cells were separated at different stages of apoptosis on the basis of cell density. Live cells that exhibited no apoptotic features floated to the top of density gradients. The most dense cells showed extensive loss of cytochrome c from mitochondria, caspase activation, chromatin condensation, and DNA fragmentation. These cells were committed to apoptosis and could not be rescued by reculturing in with nerve growth factor (NGF). Cells of intermediate density displayed no DNA fragmentation, but had begun to show cytochrome c loss, caspase activation, and chromatin condensation. This population displayed upregulation of the prodeath factor, c-Jun, and downregulation of prosurvival kinase, Akt. Importantly, apoptosis was reversible by NGF in this population. These studies suggest that increased cell density correlates with an initial step in the apoptosis mechanism that precedes irreversible commitment to suicide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:50:05