Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of hormone and growth factor on formation of fibroblast-aggregatesfor tissue-engineered skin
Autore:
Furukawa, K; Ushida, T; Kunii, K; Sakai, Y; Tanaka, J; Tateishi, T;
Indirizzi:
Univ Tokyo, Grad Sch Engn, Dept Mech Engn, Biomed Engn Tateishi & Ushida Lab, Tokyo 1138656, Japan Univ Tokyo Tokyo Japan 1138656 teishi & Ushida Lab, Tokyo 1138656, Japan Minist Int Trade & Ind, Agcy Ind Sci & Technol, Natl Inst Interdisciplinary Res, Dimens Cell & Tissue Engn Grp 3, Tsukuba, Ibaraki 305, Japan Minist Int Trade & Ind Tsukuba Ibaraki Japan 305 kuba, Ibaraki 305, Japan Univ Tokyo, Inst Ind Sci, Tokyo, Japan Univ Tokyo Tokyo JapanUniv Tokyo, Inst Ind Sci, Tokyo, Japan
Titolo Testata:
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
fascicolo: 1-2, volume: 17, anno: 2001,
pagine: 59 - 62
SICI:
0928-4931(20011101)17:1-2<59:EOHAGF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HUMAN FIBROBLASTS; DIABETIC FOOT ULCERS; DERMAL REPLACEMENT;
Keywords:
normal human skin fibroblasts; aggregates; artificial skin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Univ Tokyo, Grad Sch Engn, Dept Mech Engn, Biomed Engn Tateishi & Ushida Lab, 713 8th Bldg,Hongo 7-3-1, Tokyo 1138656, Japan Univ Tokyo 713 8th Bldg,Hongo 7-3-1 Tokyo Japan 1138656 Japan
Citazione:
K. Furukawa et al., "Effects of hormone and growth factor on formation of fibroblast-aggregatesfor tissue-engineered skin", MAT SCI E C, 17(1-2), 2001, pp. 59-62

Abstract

To accelerate wound healing after implantation of tissue-engineered skin products, higher density inoculation of fibroblasts would be important. Therefore, we attempted to develop a tissue-engineered skin with a higher density of fibroblasts by formation of cell aggregates combined with a biodegradable Polymer. Our method consisted of rotationally shaking with nontreated dishes, declining fibroblast-material interactions, and augmenting cell-cell interaction. Under the culture condition, normal neonatal human dermal fibroblasts formed spheroidal aggregates. The formation of the fibroblast-aggregates was accelerated by the addition of insulin, dexamethasone, basic fibroblast growth factor, and ascorbic acid to basal medium, and by the control of shaking time. In fibroblast-aggregates formed under a basal medium, the level of gene expression related to wound healing was higher than that of a monolayer when one added the hormones and growth factors. Therefore, tissue-engineered skin products with aggregates and scaffolds would become anartificial skin with potentiality of higher wound 'aealing. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 01:49:36