Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Deuterium NMR study of the MP-2269: albumin interaction - a step forward to the dynamics of non-covalent binding
Autore:
Adzamli, K; Vander Elst, L; Laurent, S; Muller, RN;
Indirizzi:
Mallinckrodt Inc, Imaging Div, St Louis, MO 63134 USA Mallinckrodt Inc StLouis MO USA 63134 maging Div, St Louis, MO 63134 USA Univ Mons, Dept Organ Chem, B-7000 Mons, Belgium Univ Mons Mons Belgium B-7000 ons, Dept Organ Chem, B-7000 Mons, Belgium Univ Mons, NMR Lab, B-7000 Mons, Belgium Univ Mons Mons Belgium B-7000Univ Mons, NMR Lab, B-7000 Mons, Belgium
Titolo Testata:
MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE
fascicolo: 2-3, volume: 12, anno: 2001,
pagine: 92 - 95
SICI:
0968-5243(200105)12:2-3<92:DNSOTM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTRAST AGENT; O-17 NMR; GD-DTPA; COMPLEXES; PROTEIN; MS-325;
Keywords:
NMR; non-covalent binding; MP-2269; deuterium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Adzamli, K 16212 Trade Winds Court, St Louis, MO 63040 USA 16212 Trade Winds Court St Louis MO USA 63040 is, MO 63040 USA
Citazione:
K. Adzamli et al., "Deuterium NMR study of the MP-2269: albumin interaction - a step forward to the dynamics of non-covalent binding", MAGN RES MA, 12(2-3), 2001, pp. 92-95

Abstract

MP-2269, the Gd(III) complex of 4-pentylbicyclo[2.2.2]octane-1-carboxyl-di-L-aspartyl-lysine-derived-DTPA, is a small Gd-agent that binds non-covalently to serum albumin in vivo to assume the enhanced relaxivities associatedwith macromolecular agents, (due in, part to increased rotational correlation time, tau (R)). To further explore the fundamental parameters that govern the dynamics of water proton relaxation enhancement by this prototypicalalbumin-binding agent, the rotational correlation time (tau (R)) for the deuterated La(HI) analog of MP-2269 has been independently measured in the presence and absence of 4% albumin using H-2-NMR approaches. The diamagneticLa(III) analog of MP-2269 was deuterated at the a-position of the carbonylgroups. H-2-NMR studies were conducted at 7.05T (46 MHz) and 310 degreesK on a Bruker NMR spectrometer. Spectral deconvolution permitted calculation of transverse relaxation rates, 1/T-2, from the NMR linewidths and subsequently, tau (R). The results yielded a tau (R) of the albumin bound complex of similar to8 ns. This value is intermediate between those earlier estimated by O-17-NMR (similar to1 ns) and H-1-NMRD (similar to 20-50 ns) and significantly shorter than that of albumin. The 2H-NMR study results also indicate that the exchange between free and albumin-bound forms of the La(III) analog is slow (exchange lifetimes > 1 ms). This slow exchange does not affect the water residence lifetimes (tau (M) 140-280 ns). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:57:29