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Titolo:
The effect of the arylhydrocarbon receptor on the human steroidogenic acute regulatory gene promoter activity
Autore:
Sugawara, T; Nomura, E; Sakuragi, N; Fujimoto, S;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan
Titolo Testata:
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 3, volume: 78, anno: 2001,
pagine: 253 - 260
SICI:
0960-0760(200109)78:3<253:TEOTAR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARYL-HYDROCARBON RECEPTOR; PROTEIN STAR GENE; AH-RECEPTOR; FACTOR-I; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN TCDD; INDUCIBLE EXPRESSION; ESTROGEN-RECEPTOR; GRANULOSA-CELLS; DIOXIN RECEPTOR; IDENTIFICATION;
Keywords:
arylhydrocarbon receptor; steroidogenic acute regulatory protein; StAR; Ah receptor nuclear translocator; beta-napthoflavone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, T Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Kita 15,Nishi 7, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Kita 15,Nishi 7 Sapporo Hokkaido Japan 0608638 n
Citazione:
T. Sugawara et al., "The effect of the arylhydrocarbon receptor on the human steroidogenic acute regulatory gene promoter activity", J STEROID B, 78(3), 2001, pp. 253-260

Abstract

The steroidogenic acute regulatory (StAR) protein is a rate-limiting factor in steroid hormone production. The StAR protein plays a role in the movement of cholesterol from the outer membrane to the inner membrane, where cholesterol side chain cleavage enzyme exists. Dioxins, which may act as 'endocrine disruptors', mimic and antagonize endogenous hormone actions in vivo. Although the mechanism of endocrine disruption is not clear, the actions of dioxins are known to be mediated by binding to the arylhydrocarbon receptor (AhR), and it is known that dioxins act as transcription factors to endocrine-associated gene expression. In the present study, we examined the effect of the AhR on the human StAR gene promoter, and we clarified the actionmechanisms of environmental endocrine disruptors. We transfected constructs containing the human StAR gene promoter sequences pGL(2) 1.3-kb StAR (nt - 1293 to + 39) into mouse Y-1 adrenal tumor cells and measured the promoter activity of the StAR gene. With the addition of beta -napthoflavone (betaNF), which is a ligand of AhR, to the culture medium, the activity of the StAR gene promoter increased significantly (P < 0.05), and with the addition of 1 muM of beta NF, it became maximum (3.1 +/- 0.6-fold higher than the control value). When the AhR and ARNT were co-transfected together in Y-1 cells or human adrenocortical carcinoma H295R cells, the promoter activity of the StAR gene significantly (P < 0.05) increased, to a level 1.4 +/- 0.01-fold higher in Y-1 cells and to a level 1.6 +/- 0.04-fold higher in H295R cells than the control level, when 1 muM of beta NF was added. We examined the effect of induction of cAMP with transfection with AhR or ARNT. With the addition of 1 mM 8-Br-cAMP, there were no differences between the StAR gene promoter activities in the group in which AhR and ARNT was introduced and in the group in which they were not introduced. The results suggest that AhR plays a role in the promoter activity of the human StAR gene and that the effect of AhR on StAR gene expression may cause a disturbance to the human endocrine system. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:05:09