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Titolo:
Interaction of melanin-concentrating hormone (MCH), neuropeptide E-I (NEI)9 neuropeptide G-E (NGE), and alpha-msh with melanocortin and MCH receptors on mouse B16 melanoma cells
Autore:
Hintermann, E; Tanner, H; Talke-Messerer, C; Schlumberger, S; Zumsteg, U; Eberle, AN;
Indirizzi:
Univ Basel Hosp, Dept Res ZLF, Lab Endocrinol, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 ol, CH-4031 Basel, Switzerland Univ Basel, Childrens Hosp, CH-4031 Basel, Switzerland Univ Basel Basel Switzerland CH-4031 ns Hosp, CH-4031 Basel, Switzerland
Titolo Testata:
JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH
fascicolo: 1, volume: 21, anno: 2001,
pagine: 93 - 116
SICI:
1079-9893(200102)21:1<93:IOMH(N>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; PROTEIN-COUPLED RECEPTOR; STIMULATING-HORMONE; BINDING-SITES; CYCLIC STRUCTURE; GALANIN GAL; FEMALE RAT; Y NPY; RELEASE; LEPTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
91
Recensione:
Indirizzi per estratti:
Indirizzo: Eberle, AN Univ Basel Hosp, Dept Res ZLF, Lab Endocrinol, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 Basel, Switzerland
Citazione:
E. Hintermann et al., "Interaction of melanin-concentrating hormone (MCH), neuropeptide E-I (NEI)9 neuropeptide G-E (NGE), and alpha-msh with melanocortin and MCH receptors on mouse B16 melanoma cells", J RECEPT SI, 21(1), 2001, pp. 93-116

Abstract

Melanin-concentrating hormone (MCH) and alpha -melanocyte-stimulating hormone (alpha -MSH) are known to exhibit mostly functionally antagonistic, butin some cases agonistic activities, e.g., in pigment cells and in the brain. Neuropeptide E-I (NEI) displays functional MCH-antagonist and MSH-agonist activity in different behavioral paradigms; the role of neuropeptide G-E (NGE) is not known. This study addressed the question of possible molecularinteractions between alpha -MSH, MCH and the MCH-precursor-derived peptides NEI and NGE at the level of the pigment cell MCH receptor subtype (MCH-R-pc) and the different melanocortin (MC) receptors. Radioreceptor assays using [I-125]MCH, [I-125]alpha -MSH and [I-125]NEI as radioligands and bioassays were performed with MC1-R-positive and MC1-R-negative mouse B16 melanomacells and with COS cells expressing the different MC receptors. The IC(50)s of alpha -MSH and NEI or NGE for [I-125]MCH displacement from mouse MCH-R-pc were 80-fold and, respectively, > 300-fold higher than that of MCH, andthe IC(50)s for MCH and NEI or NGE for [I-125]alpha -MSH displacement frommouse MC1-R were 50,000-fold and > 200,000-fold higher than that of alpha -MSH. No high-affinity binding sites for NEI were detected on 16 melanoma cells and there was no significant displacement of [I-125]alpha -MSH by MCH,NEI or NGE with MC3-R. MC4-R and MC5-R expressed in COS cells. At concentrations of 100 nM to 10 muM, however, MCH, NEI and NGE induced cAMP formation and melanin synthesis which could be blocked by agouti protein or inhibitors of adenylate cyclase or protein kinase A. This shows that mammalian MCH-precursor-derived peptides may mimic MSH signalling via MC1-R activation at relatively high, but physiologically still relevant concentrations, as e.g. found in autocrine/paracrine signalling mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:55:25