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Titolo:
BMCP1: a mitochondrial uncoupling protein in neurons which regulates mitochondrial function and oxidant production
Autore:
Kim-Han, JS; Reichert, SA; Quick, KL; Dugan, LL;
Indirizzi:
Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Neurol, St Louis, MO 63110 USA Washington Univ, Sch Med, Ctr Study Nervous Syst Dis, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 us Syst Dis, St Louis, MO 63110 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 3, volume: 79, anno: 2001,
pagine: 658 - 668
SICI:
0022-3042(200111)79:3<658:BAMUPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; CENTRAL-NERVOUS-SYSTEM; ENERGY-METABOLISM; SUPEROXIDE PRODUCTION; MEMBRANE; EXPRESSION; MICE; HYDROETHIDINE; CARRIER; GENE;
Keywords:
cell cultures; confocal fluorescence microscopy; dihydroethidium; free radicals; tetramethyl rhodamine ethyl ester; UCP5;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Dugan, LL Washington Univ, Sch Med, Dept Neurol, 660 S Euclid Ave,Box 8111, St Louis, MO 63110 USA Washington Univ 660 S Euclid Ave,Box 8111 St LouisMO USA 63110
Citazione:
J.S. Kim-Han et al., "BMCP1: a mitochondrial uncoupling protein in neurons which regulates mitochondrial function and oxidant production", J NEUROCHEM, 79(3), 2001, pp. 658-668

Abstract

Outside the nervous system, members of the mitochondrial uncoupling protein (UCP) family have been proposed to contribute to control of body temperature and energy metabolism, and regulation of mitochondrial production of reactive oxygen species (ROS). However, the function of brain mitochondrial carrier protein 1 (BMCP1), which is highly expressed in brain, remains to bedetermined. To study BMCP1 expression and function in the nervous system, a high-affinity antibody to BMCP1 was generated and used to analyze tissue expression of BMCP1 protein in mouse. BMCP1 protein was highly expressed inheart and kidney, but not liver or lung. In the nervous system, BMCP1 was present in cortex, basal ganglia, substantia nigra, cerebellum, and spinal cord. Both BMCP1 mRNA and protein xpression was almost exclusively neuronal. To study the effect of BMCP1 expression on mitochondrial function, neuronal (GT1-1) cell lines with stable overexpression of BMCP1 were generated. Transfected cells had higher State 4 respiration and lower mitochondrial membrane potential (psi (m)), consistent with greater mitochondrial uncoupling. BMCP1 expression also decreased mitochondrial production of ROS. These data suggest that BMCP1 can modify mitochondrial respiratory efficiency and mitochondrial oxidant production, and raise the possibility that BMCP1 mightalter the vulnerability of brain to both acute injury and to neurodegenerative conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:29:58