Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Sustained release of granulocyte-macrophage colony-stimulating factor froma modular peptide-based cancer vaccine alters vaccine microenvironment andenhances the antigen-specific T-cell response
Autore:
Nguyen, CL; Bui, JT; Demcheva, M; Vournakis, JN; Cole, DJ; Gillanders, WE;
Indirizzi:
Med Univ S Carolina, Dept Surg, Sect Surg Oncol, Charleston, SC 29425 USA Med Univ S Carolina Charleston SC USA 29425 col, Charleston, SC 29425 USA Hollings Canc Ctr, Ctr Struct & Mol Biol, Charleston, SC USA Hollings CancCtr Charleston SC USA truct & Mol Biol, Charleston, SC USA
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 5, volume: 24, anno: 2001,
pagine: 420 - 429
SICI:
1524-9557(200109/10)24:5<420:SROGCF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECRUITS DENDRITIC CELLS; IN-VIVO; METASTATIC MELANOMA; MANNOSE RECEPTOR; HOST-DEFENSE; INDUCTION; TUMOR; IMMUNITY; MICE; GLUCOSAMINE;
Keywords:
vaccination; granulocyte-macrophage colony-stimulating; factor; dendritic cells; cytotoxic T lymphocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Gillanders, WE Med Univ S Carolina, Dept Surg, Sect Surg Oncol, Room 420P,96 Jonathan Lucas St,POB 250613, Charleston, SC 29425 USA Med Univ S Carolina Room 420P,96 Jonathan Lucas St,POB 250613 Charleston SC USA 29425
Citazione:
C.L. Nguyen et al., "Sustained release of granulocyte-macrophage colony-stimulating factor froma modular peptide-based cancer vaccine alters vaccine microenvironment andenhances the antigen-specific T-cell response", J IMMUNOTH, 24(5), 2001, pp. 420-429

Abstract

The recent identification and molecular characterization of tumor antigensprovides the opportunity to explore the rational development of peptide-based cancer vaccines, However. the response to these vaccines remains variable, and peptide-based cancer vaccines may even produce tolerance induction and enhanced tumor growth. The authors have developed a unique method for the isolation of a polysaccharide polymer of chemically pure poly-N-acetyl glucosamine (p-GIcNAc). This highly purified polysaccharide can be formulated into a stable gel matrix (designated F2 gel matrix) with unique properties of a sustained-re lease delivery system that has previously been shown tobe an effective immune adjuvant. F2 gel matrix is capable of providing sustained release of antigenic peptide and cytokine in vitro. The purposes of this study were to characterize the ability of F2 gel matrix to provide sustained local release of cytokines in vivo and to test the hypothesis that such sustained release can enhance th microenvironment for antigen presentation, leading to a more effective antitumor response. Subcutaneous administration of F2 gel/cytokine matrix resulted in sustained release of cytokine at the vaccine site for up to 120 hours. Sustained release of granulocyte-macrophage colony-stimulating factor (GM-CSF) was associated with an increased inflammatory infiltrate at the vaccine site and enhanced dendritic cell activation. Further, accination with F2 gel/SIINFEKL/GM-CSF matrix resulted in enhanced antigen-specific immunity. Addition of GM-CSF to the F2 gel matrix resulted in an increase in the percentage of antigen-specific T cells in the draining lymph nodes, enhanced cytotoxicity, a sustained presence of antigen-specific T cells in the peripheral blood, and protection from E.G7 tumor challenge. These results support the potential of an F2 gel matrix modular vaccine delivery system that can provide sustained local release of cytokine in vivo, and confirm the powerful effects of GM-CSF as an immune adjuvant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:48:44