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Titolo:
Lipopolysaccharides from distinct pathogens induce different classes of immune responses in vivo
Autore:
Pulendran, B; Kumar, P; Cutler, CW; Mohamadzadeh, M; Van Dyke, T; Banchereau, J;
Indirizzi:
Baylor Inst Immunol Res, Dallas, TX 75204 USA Baylor Inst Immunol Res Dallas TX USA 75204 nol Res, Dallas, TX 75204 USA SUNY Stony Brook, Sch Dent Med, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 Med, Stony Brook, NY 11794 USA Boston Univ, Sch Med, Dept Periodontol & Oral Biol, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 odontol & Oral Biol, Boston, MA 02118 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 9, volume: 167, anno: 2001,
pagine: 5067 - 5076
SICI:
0022-1767(20011101)167:9<5067:LFDPID>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-HELPER CELL; PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE; INTERFERON-GAMMA PRODUCTION; TOLL-LIKE RECEPTOR-2; DEFINED LIPID-A; IN-VIVO; DENDRITIC CELLS; CLONAL EXPANSION; C3H/HEJ MICE; WALL COMPONENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Pulendran, B Aventis Pharmaceut, Route 202-206,POB 6800, Bridgewater, NJ 08807 USA Aventis Pharmaceut Route 202-206,POB 6800 Bridgewater NJ USA 08807
Citazione:
B. Pulendran et al., "Lipopolysaccharides from distinct pathogens induce different classes of immune responses in vivo", J IMMUNOL, 167(9), 2001, pp. 5067-5076

Abstract

The adaptive immune system has evolved distinct responses against different pathogens, but the mechanism(s) by which a particular response is initiated is poorly understood. In this study, we investigated the type of Ag-specific CD4(+) Th and CD8(+) T cell responses elicited in vivo, in response tosoluble OVA, coinjected with LPS from two different pathogens. We used Escherichia coli LPS, which signals through Toll-like receptor 4 (TLR4) and LPS from the oral pathogen Porphyromonas gingivalis, which does not appear torequire TLR4 for signaling. Coinjections of E. coli LPS + OVA or P. gingivalis LPS + OVA induced similar clonal expansions of OVA-specific CD4+ and CD8+ T cells, but strikingly different cytokine profiles. E. coli LPS induced a Th1-like response with abundant IFN-gamma, but little or no IL-4, IL-13, and IL-5. In contrast, P. gingivalis LPS induced Th and T cell responses characterized by significant levels of IL-13, IL-5, and IL-10, but lower levels of IFN-gamma. Consistent with these results, E. coli LPS induced IL-12(p70) in the CD8 alpha (+) dendritic cell (DC) subset, while P. gingivalis LPS did not. Both LPS, however, activated the two DC subsets to up-regulatecostimulatory molecules and produce IL-6 and TNF-alpha. Interestingly, these LPS appeared to have differences in their ability to signal through TLR4; proliferation of splenocytes and cytokine secretion by splenocytes or DCsfrom TLR4-deficient C3H/HeJ mice were greatly impaired in response to E. coli LPS, but not P. gingivalis LPS. Therefore, LPS from different bacteria activate DC subsets to produce different cytokines, and induce distinct types of adaptive immunity in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 16:33:23