Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cutting edge: Neosynthesis is required for the presentation of a T cell epitope from a long-lived viral protein
Autore:
Khan, S; de Giuli, R; Schmidtke, G; Bruns, M; Buchmeier, M; van den Broek, M; Groettrup, M;
Indirizzi:
Kantonsspital, Lab Forsch Abt, Res Dept, CH-9007 St Gallen, Switzerland Kantonsspital St Gallen Switzerland CH-9007 -9007 St Gallen, Switzerland Univ Hamburg, Heinrich Pette Inst Expt Virol & Immunol, D-2000 Hamburg, Germany Univ Hamburg Hamburg Germany D-2000 l & Immunol, D-2000 Hamburg, Germany Scripps Clin, Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA Scripps Clin La Jolla CA USA 92037 Neuropharmacol, La Jolla, CA 92037 USA Univ Zurich Hosp, Dept Pathol, Inst Expt Immunol, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 9, volume: 167, anno: 2001,
pagine: 4801 - 4804
SICI:
0022-1767(20011101)167:9<4801:CENIRF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEWLY SYNTHESIZED PROTEINS; DEGRADATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Groettrup, M Kantonsspital, Lab Forsch Abt, Res Dept, Haus 09, CH-9007 St Gallen, Switzerland Kantonsspital Haus 09 St Gallen Switzerland CH-9007 tzerland
Citazione:
S. Khan et al., "Cutting edge: Neosynthesis is required for the presentation of a T cell epitope from a long-lived viral protein", J IMMUNOL, 167(9), 2001, pp. 4801-4804

Abstract

CTLs recognize peptide epitopes which are proteolytically generated by theproteasome and presented on MHC class I molecules. According to the defective ribosomal product (DRIP) hypothesis, epitopes originate from newly synthesized polypeptides which are degraded shortly after their translation. The DRIP hypothesis would explain how epitopes can be generated from long-lived proteins. We examined whether neosynthesis is required for presentation of the immunodominant epitope NP118 of the lymphocytic choriomeningitis virus nucleoprotein, which has a half-life of >3 days. Two days after nucleoprotein biosynthesis was terminated in a tetracycline-regulated transfectant,the presentation of the NP118 epitope ceased. This indicates that NP118 epitopes are generated from newly synthesized nucleoproteins rather than fromthe long-lived pool of nucleoproteins in the cell. Therefore, the lymphocytic choriomeningitis virus nucleoprotein is the first substrate for which amajor prediction of the DRIP hypothesis, namely the requirement for neosynthesis, is shown to hold true.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:53:19