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Titolo:
A new allele of the short tandem repeat (STR) locus, CSF1PO
Autore:
Margolis-Nunno, H; Brenner, L; Cascardi, J; Kobilinsky, L;
Indirizzi:
CUNY John Jay Coll Criminal Justice, Dept Sci, New York, NY 10019 USA CUNYJohn Jay Coll Criminal Justice New York NY USA 10019 k, NY 10019 USA Philadelphia Police Dept, DNA Identificat Lab, Philadelphia, PA USA Philadelphia Police Dept Philadelphia PA USA t Lab, Philadelphia, PA USA
Titolo Testata:
JOURNAL OF FORENSIC SCIENCES
fascicolo: 6, volume: 46, anno: 2001,
pagine: 1480 - 1483
SICI:
0022-1198(200111)46:6<1480:ANAOTS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA;
Keywords:
forensic science; DNA typing; short tandem repeats; CSF1PO; variants; alleles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Margolis-Nunno, H CUNY John Jay Coll Criminal Justice, Dept Sci, 899 10th Ave, New York, NY 10019 USA CUNY John Jay Coll Criminal Justice 899 10th Ave New York NY USA 10019
Citazione:
H. Margolis-Nunno et al., "A new allele of the short tandem repeat (STR) locus, CSF1PO", J FOREN SCI, 46(6), 2001, pp. 1480-1483

Abstract

CSF1PO is one of the thirteen core loci used for the CODIS database, and alleles reported for this short tandem repeat (STR) locus contain from 6 to 15 repeats of the tetranucleotide AGAT. Screening of DNA from 76 individuals by gel electrophoresis and silver stain detection yielded one sample thatcontained a rare, off-ladder CSF1PO allele; an allele larger than CSF1PO(15) was detected in a heterozygote that also contained a CSF1PO(10) allele. Capillary electrophoresis analysis using Gene-Scan (TM) software demonstrated that the variant allele contained four bases more than CSF1PO(15). Following agarose get electrophoresis to separate the two alleles of the heterozygote and cycle sequencing using dye terminators, sequence analysis showed that the variant, which was otherwise identical to the CSF1PO GenBank sequence, contained exactly 16 AGAT repeats. These results demonstrate the existence of an additional CSF1PO allele, a previously unreported size variant.,CSF1PO(16).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:29:33