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Titolo:
A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films
Autore:
Piro, B; Dang, LA; Pham, MC; Fabiano, S; Tran-Minh, C;
Indirizzi:
Univ Paris 07, Inst Topol & Dynam Syst, CNRS, URA 34, F-75005 Paris, France Univ Paris 07 Paris France F-75005 , CNRS, URA 34, F-75005 Paris, France Ecole Natl Super Mines, Ctr SPIN, F-42023 St Etienne Du Rouvray 2, France Ecole Natl Super Mines St Etienne Du Rouvray France 2 Rouvray 2, France
Titolo Testata:
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
fascicolo: 1-2, volume: 512, anno: 2001,
pagine: 101 - 109
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT ELECTRON-TRANSFER; ELECTROCHEMICAL IMMOBILIZATION; CONDUCTING POLYMERS; POLYPYRROLE FILMS; OXIDASE ELECTRODE; SENSOR; ENTRAPMENT; SURFACES; STABILITY; MEMBRANE;
Keywords:
biosensors; covalent immobilisation; conducting polymers; glucose; electrochemical quartz microbalance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Pham, MC Univ Paris 07, Inst Topol & Dynam Syst, CNRS, URA 34, 1 Rue Guy de la Brosse, F-75005 Paris, France Univ Paris 07 1 Rue Guy de la Brosse Paris France F-75005 France
Citazione:
B. Piro et al., "A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films", J ELEC CHEM, 512(1-2), 2001, pp. 101-109

Abstract

We have constructed and characterised a glucose sensor using glucose oxidase (GOD) covalently attached to carboxylic acid polyethyleneglycol (PEG), called (PEG-GOD). This modified enzyme was entrapped afterwards within poly(3,4-ethylenedioxythiophene) (PEDT) films electrogenerated on glassy carbon (GC) electrodes. The composite (PEG-GOD/PEDT) film is more porous than the film without enzyme (PEDT + PEG). Data from electrochemical quartz microbalance (ECQM) and pH-stat experiments indicate a good relative activity of the modified enzyme, ca. 12-15%. Amperometric measurements, using ferrocenemethanol as the redox mediator, confirms that the modified enzyme is catalytically active. The effect of film thickness was also investigated. The sensitivities were quite similar for modified-GOD electrodes (ca. 3 mA cm (-2) M(-1)) and unmodified-GOD electrodes (ca. 2.7 mA cm -(2) M (-1)) but a better stability was obtained with modified PEG-GOD electrodes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:16:28