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Titolo:
Western blot analysis of sera reactive to human monocytic ehrlichiosis andhuman granulocytic ehrlichiosis agents
Autore:
Unver, A; Felek, S; Paddock, CD; Zhi, N; Horowitz, HW; Wormser, GP; Cullman, LC; Rikihisa, Y;
Indirizzi:
Ohio State Univ, Coll Vet Med, Dept Vet Biosci, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Vet Biosci, Columbus, OH 43210 USA Ctr Dis Control & Prevent, Viral & Rickettsial Zoonoses Branch, Atlanta, GA 30333 USA Ctr Dis Control & Prevent Atlanta GA USA 30333 nch, Atlanta, GA 30333 USA New York Med Coll, Dept Med, Div Infect Dis, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 nfect Dis, Valhalla, NY 10595 USA MRL Ref Lab, Cypress, CA 90630 USA MRL Ref Lab Cypress CA USA 90630MRL Ref Lab, Cypress, CA 90630 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 11, volume: 39, anno: 2001,
pagine: 3982 - 3986
SICI:
0095-1137(200111)39:11<3982:WBAOSR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; NEW-YORK-STATE; IMMUNOBLOT ANALYSIS; CHAFFEENSIS; CANIS; INFECTION; PROTEINS; IDENTIFICATION; SERODIAGNOSIS; EWINGII;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Rikihisa, Y Ohio State Univ, Coll Vet Med, Dept Vet Biosci, 1925 Coffey Rd, Columbus, OH 43210 USA Ohio State Univ 1925 Coffey Rd Columbus OH USA 43210 43210 USA
Citazione:
A. Unver et al., "Western blot analysis of sera reactive to human monocytic ehrlichiosis andhuman granulocytic ehrlichiosis agents", J CLIN MICR, 39(11), 2001, pp. 3982-3986

Abstract

Laboratory diagnosis of human ehrlichioses is routinely made by an indirect immunofluorescence assay (IFA) using cultured ehrlichia-infected whole cells as antigen. Concern has been raised that incorrect diagnoses of human monocytic ehrlichiosis (HME) or human granulocytic ehrlichiosis (HGE) may bemade on the basis of serologic cross-reactivity between Ehrlichia chaffeensis and the agent of HGE. The present study examined whether two recombinant major outer membrane proteins, rP30 and rP44, that were previously shown to be sensitive and specific serodiagnostic antigens for HME and HGE, respectively, could be used to discriminate IFA dually reacting sera. Thirteen dually IFA-reactive sera, three sera that were IFA positive only with E. chaffeensis, and three sera that were IFA positive only with the HGE agent were examined by Western immunoblot analysis using purified whole organisms and recombinant proteins as antigens. All 16 E. chaffeensis IFA-positive serareacted with rP30. However, none of these sera reacted with rP44, regardless of IFA reactivity with the HGE agent. The three HGE-agent-only IFA-positive sera reacted only with rP44, not with rP30. Western immunoblotting using purified E. chaffeensis and the HGE agent as antigens suggested that heatshock and other proteins, but not major outer membrane proteins, cross-react between the two organisms. Therefore, Western immunoblot analysis using rP44 and rP30 may be useful in discriminating dually HME and HGE IFA-reactive sera.

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Documento generato il 10/07/20 alle ore 18:23:57