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Titolo:
Centrosome cohesion is regulated by a balance of kinase and phosphatase activities
Autore:
Meraldi, P; Nigg, EA;
Indirizzi:
Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 Martinsried, Germany
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 20, volume: 114, anno: 2001,
pagine: 3749 - 3757
SICI:
0021-9533(200110)114:20<3749:CCIRBA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS EGG EXTRACTS; NEK2 PROTEIN-KINASE; CELL-CYCLE; SELF-ORGANIZATION; MAMMALIAN-CELLS; MITOTIC SPINDLE; OKADAIC ACID; HELA-CELLS; PHOSPHORYLATION; DUPLICATION;
Keywords:
cell cycle; centriole; Nek2; PP1 alpha; phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Nigg, EA Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 ed, Germany
Citazione:
P. Meraldi e E.A. Nigg, "Centrosome cohesion is regulated by a balance of kinase and phosphatase activities", J CELL SCI, 114(20), 2001, pp. 3749-3757

Abstract

Centrosome cohesion and separation are regulated throughout the cell cycle, but the underlying mechanisms are not well understood. Since overexpression of a protein kinase, Nek2, is able to trigger centrosome splitting (the separation of parental centrioles), we have surveyed a panel of centrosome-associated kinases for their ability to induce a similar phenotype. Cdk2, in association with either cyclin A or E, was as effective as Nek2, but several other kinases tested did not significantly interfere with centrosome cohesion. Centrosome splitting could also be triggered by inhibition of phosphatases, and protein phosphatase 1 alpha (PP1 alpha) was identified as a likely physiological antagonist of Nek2. Furthermore, we have revisited the role of the microtubule network in the control of centrosome cohesion. We could confirm that microtubule depolymerization by nocodazole causes centrosome splitting. Surprisingly, however, this drug-induced splitting also required kinase activity and could specifically be suppressed by a dominant-negative mutant of Nek2. These studies highlight the importance of protein phosphorylation in the control of centrosome cohesion, and they point to Nek2 and PP1 alpha as critical regulators of centrosome structure.

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Documento generato il 02/04/20 alle ore 02:36:57