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Titolo:
Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex
Autore:
Sprong, H; Degroote, S; Claessens, T; van Drunen, J; Oorschot, V; Westerink, BHC; Hirabayashi, Y; Klumperman, J; van der Sluijs, P; van Meer, G;
Indirizzi:
Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, NL-1100 DE Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1100 DE E Amsterdam, Netherlands Univ Utrecht, Med Ctr, Dept Cell Biol, Inst Biomembranes, NL-3584 CX Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CX 3584 CX Utrecht, Netherlands Ctr Biomed Genet, NL-3584 CX Utrecht, Netherlands Ctr Biomed Genet Utrecht Netherlands NL-3584 CX CX Utrecht, Netherlands Univ Groningen, Ctr Pharm, Dept Med Chem, NL-9713 AV Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 AV V Groningen, Netherlands RIKEN, Lab Memory & Learning, Brain Sci Inst, Wako, Saitama 3510198, JapanRIKEN Wako Saitama Japan 3510198 n Sci Inst, Wako, Saitama 3510198, Japan
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 3, volume: 155, anno: 2001,
pagine: 369 - 379
SICI:
0021-9525(20011029)155:3<369:GARFSM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINASE-RELATED PROTEIN-1; MOUSE MELANOMA-CELLS; DI-LEUCINE; MDCK CELLS; CERAMIDE GALACTOSYLTRANSFERASE; CYTOPLASMIC TAIL; EPITHELIAL-CELLS; MEMBRANE RAFTS; AP-3 ADAPTER; TRANSPORT;
Keywords:
glycosphingolipid; protein sorting; melanosome; tyrosinase; TRP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: van Meer, G Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, POB 22700, NL-1100 DE Amsterdam, Netherlands Univ Amsterdam POB 22700 Amsterdam Netherlands NL-1100 DE nds
Citazione:
H. Sprong et al., "Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex", J CELL BIOL, 155(3), 2001, pp. 369-379

Abstract

Although glycosphingolipids are ubiquitously expressed and essential for multicellular organisms, surprisingly little is known about their intracellular functions. To explore the role of glycosphingolipids in membrane transport, we used the glycosphingolipid-deficient GM95 mouse melanoma cell line. We found that GM95 cells do not make melanin pigment because tyrosinase, the first and rate-limiting enzyme in melanin synthesis, was not targeted tomelanosomes but accumulated in the Golgi complex. However, tyrosinase-related protein 1 still reached melanosomal structures via the plasma membrane instead of the direct pathway from the Golgi. Delivery of lysosomal enzymesfrom the Golgi complex to endosomes was normal, suggesting that this pathway is not affected by the absence of glycosphingolipids. Loss of pigmentation was due to tyrosinase mislocalization, since transfection of tyrosinase with an extended transmembrane domain, which bypassed the transport block, restored pigmentation. Transfection of ceramide glucosyltransferase or addition of glucosylsphingosine restored tyrosinase transport and pigmentation. We conclude that protein transport from Golgi to melanosomes via the direct pathway requires glycosphingolipids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:25:57