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Titolo:
Different subtypes of alpha(1)-adrenoceptor modulate different K+ currentsvia different signaling pathways in canine ventricular myocytes
Autore:
Wang, HZ; Yang, BF; Zhang, YQ; Han, H; Wang, JX; Shi, H; Wang, ZG;
Indirizzi:
Montreal Heart Inst, Res Ctr, Montreal, PQ H1T 1C8, Canada Montreal Heart Inst Montreal PQ Canada H1T 1C8 ntreal, PQ H1T 1C8, Canada Univ Montreal, Dept Med, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ed, Montreal, PQ H3C 3J7, Canada Harbin Med Coll, Dept Pharmacol, Harbin 150086, Heilongjiang, Peoples R China Harbin Med Coll Harbin Heilongjiang Peoples R China 150086 eoples R China
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 44, volume: 276, anno: 2001,
pagine: 40811 - 40816
SICI:
0021-9258(20011102)276:44<40811:DSOAMD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT OUTWARD CURRENT; HUMAN ATRIAL MYOCYTES; PROTEIN-KINASE; PURKINJE-FIBERS; CHANNEL; STIMULATION; MECHANISMS; MYOCARDIUM; AGONISTS; ANTAGONISTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, ZG Montreal Heart Inst, Res Ctr, 500 Belanger E, Montreal, PQ H1T 1C8, Canada Montreal Heart Inst 500 Belanger E Montreal PQ Canada H1T 1C8 ada
Citazione:
H.Z. Wang et al., "Different subtypes of alpha(1)-adrenoceptor modulate different K+ currentsvia different signaling pathways in canine ventricular myocytes", J BIOL CHEM, 276(44), 2001, pp. 40811-40816

Abstract

Multiple subtypes (alpha (1A), alpha (1B), and alpha (1D)) of alpha (1)-adrenoreceptors (alpha (1)ARs) co-exist in the heart and mediate a variety ofcellular functions. We studied alpha (1)AR modulation of inward rectifier (I-K1) and transient outward (I-to) K+ currents in canine ventricular myocytes. Phenylephrine at 10 mum depressed only It. without affecting I-K1 and at 100 muM inhibited both I-to and I-K1. The effect of phenylephrine on I-to. was abolished by (+)niguldipine (10 nm) to inhibit alpha (1A)ARs but notby chloroethyclonidine (10 mum) to inactivate alpha (1B)ARs nor by BMY-7378 to antagonize alpha (1D)ARs. In contrast, phenylephrine inhibition of I-K1 was reversed only by BMY-7378 (1 nM). PDD (100 nM, phorbol ester activator of protein kinase C (PKC)) simulates and bisindolylmaleimide (50 nM, PKC inhibitor) weakens phenylephrine modulation of I-to. but not I-K1. Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor KN-93 and inhibitor peptides abolished the effects of phenylephrine on I-K1. Enhancement of PKCor CaMKII activities was seen in alpha (1a)AR- or alpha (1d)dAR-transfected HEK293 cells and in myocytes pretreated with 10 or 100 muM phenylephrine,respectively. Our data suggest that different subtypes of alpha 1ARs selectively modulate different cardiac K+ currents via different signal transduction mechanisms; alpha (1A)ARs mediate It. regulation via PKC, and alpha (1D)ARs mediate I-K1 regulation via CaMKII.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:07:31