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Titolo:
Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion
Autore:
Woolhead, CA; Thompson, SJ; Moore, M; Tissier, C; Mant, A; Rodger, A; Henry, R; Robinson, C;
Indirizzi:
Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA Univ Arkansas Fayetteville AR USA 72701 l Sci, Fayetteville, AR 72701 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 44, volume: 276, anno: 2001,
pagine: 40841 - 40846
SICI:
0021-9258(20011102)276:44<40841:DAA-PF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-RECOGNITION PARTICLE; ESCHERICHIA-COLI; POSTTRANSLATIONAL INTEGRATION; PHOTOSYSTEM-II; INNER MEMBRANE; SEC; TRANSPORT; BINDING; HOMOLOG; OXA1P;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL nds, England
Citazione:
C.A. Woolhead et al., "Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion", J BIOL CHEM, 276(44), 2001, pp. 40841-40846

Abstract

The homologous proteins Oxa1, YidC, and Alb3 mediate the insertion of membrane proteins in mitochondria, bacteria, and chloroplast thylakoids, respectively. Depletion of YidC in Escherichia coli affects the integration of every membrane protein studied, and Alb3 has been shown previously to be required for the insertion of a signal recognition particle (SRP)-dependent protein, Lhcb1, in thylakoids. In this study we have analyzed the "global" role of Alb3 in the insertion of thylakoid membrane proteins. We show that insertion of two chlorophyll-binding proteins, Lhcb4.1 and Lhcb5, is almost totally blocked by preincubation of thylakoids with anti-Alb3 antibodies, indicating a requirement for Alb3 in the insertion pathway. Insertion of the related PsbS protein, on the other hand, is unaffected by Alb3 antibodies, and insertion of a group of SRP-independent, signal peptide-bearing proteins, PsbX, PsbW, and PsbY, is likewise completely unaffected. Proteinase K is furthermore able to completely degrade Alb3, but this treatment does not affect the insertion of these proteins. Among the thylakoid proteins studied here, Alb3 requirement correlates strictly with a requirement for stromal factors and nucleoside triphosphates. However, the majority of proteins tested do not require Alb3 or any other known form of translocation apparatus.

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Documento generato il 09/04/20 alle ore 19:46:03