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Titolo:
Free fatty acids activate a vigorous Ca2+: 2H(+) antiport activity in yeast mitochondria
Autore:
Bradshaw, PC; Jung, DW; Pfeiffer, DR;
Indirizzi:
Ohio State Univ, Dept Mol & Cellular Biochem, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 lar Biochem, Columbus, OH 43210 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 44, volume: 276, anno: 2001,
pagine: 40502 - 40509
SICI:
0021-9258(20011102)276:44<40502:FFAAAV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY TRANSITION PORE; SACCHAROMYCES-CEREVISIAE MITOCHONDRIA; RAT-LIVER MITOCHONDRIA; INORGANIC PYROPHOSPHATASE; ADENINE-NUCLEOTIDES; IONOPHORE A23187; CA2+ TRANSPORT; CALCIUM; MEMBRANE; PHOSPHOLIPASE-A2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Pfeiffer, DR Ohio State Univ, Dept Mol & Cellular Biochem, 1645 Neil Ave,Hamilton Hall 310A, Columbus, OH 43210 USA Ohio State Univ 1645 Neil Ave,Hamilton Hall 310A Columbus OH USA 43210
Citazione:
P.C. Bradshaw et al., "Free fatty acids activate a vigorous Ca2+: 2H(+) antiport activity in yeast mitochondria", J BIOL CHEM, 276(44), 2001, pp. 40502-40509

Abstract

The accumulation and retention of Ca2+ by yeast mitochondria (Saccharomyces cerevisiae) mediated by ionophore ETH 129 occurs with a variable efficiency in different preparations. Ineffective Ca2+ transport and a depressed membrane potential occur in parallel, are exacerbated in parallel by exogenous free fatty acids, and are corrected in parallel by the addition of bovineserum albumin. Bovine serum albumin is not required to develop a high membrane potential when either Ca2+ or ETH 129 are absent, and when both are present membrane potential is restored by the addition of EGTA in a concentration-dependant manner. Respiration and swelling data indicate that the permeability transition pore does not open in yeast mitochondria that are treated with Ca2+ and ETH 129, whereas fatty acid concentration studies and the inaction of carboxyatractyloside indicate that fatty acid-derived uncoupling does not underlie the other observations. It is concluded that yeast mitochondria contain a previously unrecognized Ca2+:2H(+) antiporter that is highly active in the presence of free fatty acids and leads to a futile cycleof Ca2+ accumulation and release when exogenous Ca2+ and ETH 129 are available. It is also shown that isolated yeast mitochondria degrade their phospholipids at a relatively rapid rate. The activity responsible is also previously unrecognized. It is Ca2+-independent, little affected by the presenceor absence of a respiratory substrate, and leads to the hydrolysis of ester linkages at both the sn-1 and sn-2 positions of the glycerophospholipids. The products of this activity, through their actions on the antiporter, explain the variable behavior of yeast mitochondria treated with Ca2+ plus ETH 129.

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Documento generato il 14/07/20 alle ore 13:17:48