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Titolo:
Bivalent metal ions modulate Cd2+ effects on isolated rat liver mitochondria
Autore:
Belyaeva, EA; Glazunov, VV; Nikitina, ER; Korotkov, SM;
Indirizzi:
Russian Acad Sci, IM Sechenov Evolutionary Physiol & Biochem Inst, St Petersburg 194223, Russia Russian Acad Sci St Petersburg Russia 194223 t Petersburg 194223, Russia
Titolo Testata:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
fascicolo: 4, volume: 33, anno: 2001,
pagine: 303 - 318
SICI:
0145-479X(200108)33:4<303:BMIMCE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA-2+-INDUCED MEMBRANE TRANSITION; OXIDATIVE-PHOSPHORYLATION; DIVALENT-CATIONS; LIPID-PEROXIDATION; ELECTRON-TRANSPORT; CALCIUM-TRANSPORT; CADMIUM; HEPATOCYTES; INHIBITION; PERMEABILITY;
Keywords:
Cd2+; bivalent metal ions; ruthenium red; mitochondrial respiration; mitochondrial swelling; uptake of (CS)-C-137; mitochondrial permeability transition; ion transport; inner mitochondrial membrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Korotkov, SM Russian Acad Sci, IM Sechenov Evolutionary Physiol & Biochem Inst, Thorez Pr 44, St Petersburg 194223, Russia Russian Acad Sci Thorez Pr44 St Petersburg Russia 194223 ia
Citazione:
E.A. Belyaeva et al., "Bivalent metal ions modulate Cd2+ effects on isolated rat liver mitochondria", J BIOENER B, 33(4), 2001, pp. 303-318

Abstract

We have studied Cd2+-induced effects on mitochondrial respiration and swelling in various media as a function of the [Cd2+] in the presence or absence of different bivalent metal ions or ruthenium red (RR). It was confirmed by monitoring oxygen consumption by isolated rat liver mitochondria that, beginning from 5 muM, Cd2+ decreased both ADP and uncoupler-stimulated respiration and increased their basal respiration when succinate was used as respiratory substrate. At concentrations higher than 5 muM, Cd2+ stimulated ion permeability of the inner mitochondrial membrane, which was monitored in this study by swelling of both nonenergized mitochondria in 125 mM KNO3 Or NH4NO3 medium and succinate-energized mitochondria incubated in a medium containing 25 mM K-acetate and 100 mM sucrose. We have found substantial changes in the above-mentioned Cd2+ effects on mitochondria treated in sequencewith 100 muM of Ca2+, Sr2+, Mn2+ or Ba2+(Me2+) and 7.5 muM RR, as well as the alterations in Cd2+ action on the uptake of Cs-137(+) by succinate-energized mitochondria in the presence or absence of valinomycin in acetate medium (50 mM Tris-acetate and 140 mM sucrose) with or without Ca2+ or RR. Theevidence obtained indicate that Ca2+ exhibits a synergestic action on all Cd2+ effects examined, whereas Sr2+ and Mn2+ conversely, are antagonistic. In the presence of`RR, the Cd2+ effects on respiration [stimulation ofState4 respiration and inhibition of 2,4-dinitrophenol (DNP)-uncoupled respiration] still exist, but are observed at concentrations of cadmium more than one order higher; the inhibition of State 3 respiration by Cd2+ conversely, takes place under even lower cadmium concentrations than those determined without RR in the medium. In addition, RR added simultaneously with cadmium in the incubation medium prevents any swelling in the nitrate media, but induces an increment both in Cd2+-stimulated swelling and Cs-137(+) (analog of K+) uptake in the acetate media. For the first time, we have shown that Cd2+-induced swelling in all media under study is susceptible to cyclosporinA (CSA), a high-potency inhibitor of the mitochondrial permeability transition (PT) pore. The observations are interpreted in terms of a dual effect of cadmium on respiratory chain activity and permeability transition.

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Documento generato il 11/07/20 alle ore 16:23:05