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Titolo:
Differential effect of PKC isoform on insulin- and phorbol ester-stimulated glucose uptake mechanism in rat adipocytes
Autore:
Ishizuka, T; Miura, A; Kajita, K; Ishizawa, M; Kimura, M; Huang, Y; Kawai, Y; Morita, H; Uno, Y; Yasuda, K;
Indirizzi:
Gifu Univ, Sch Med, Dept Gen Med, Gifu 5008705, Japan Gifu Univ Gifu Japan 5008705 Sch Med, Dept Gen Med, Gifu 5008705, Japan Gifu Univ, Sch Med, Dept Internal Med 3, Gifu 5008705, Japan Gifu Univ Gifu Japan 5008705 d, Dept Internal Med 3, Gifu 5008705, Japan
Titolo Testata:
IUBMB LIFE
fascicolo: 5, volume: 51, anno: 2001,
pagine: 299 - 304
SICI:
1521-6543(200105)51:5<299:DEOPIO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; PHOSPHATIDYLINOSITOL 3-KINASE; TYROSINE PHOSPHORYLATION; RECEPTOR SUBSTRATE-1; GLYCOGEN-SYNTHESIS; 3T3-L1 ADIPOCYTES; SOLEUS MUSCLES; POTENTIAL ROLE; ACTIVATION; TRANSPORT;
Keywords:
glucose uptake; insulin PI 3-kinase; phorbol ester; PKC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ishizuka, T Gifu Univ, Sch Med, Dept Gen Med, Tsukasamachi 40, Gifu 5008705, Japan Gifu Univ Tsukasamachi 40 Gifu Japan 5008705 u 5008705, Japan
Citazione:
T. Ishizuka et al., "Differential effect of PKC isoform on insulin- and phorbol ester-stimulated glucose uptake mechanism in rat adipocytes", IUBMB LIFE, 51(5), 2001, pp. 299-304

Abstract

Insulin stimulates glucose uptake in association with phosphatidylinositol(PI) 3-kinase activation mechanisms in rat adipocytes. Insulin stimulated glucose uptake to 6.5-fold, and 12-o-tetradecanoyl phorbol 13-acetate (TPA)also stimulated glucose uptake to 4.5-fold in rat adipocytes. We examined these differences in glucose uptake, PKC zeta activation, and PI 3-kinase activation after stimulation with insulin and TPA. TPA stimulated PI 3-kinase activity and increased the p85 subunit of PI 3-kinase immunoreactivity inanti-phosphotyrosine antibody-immunoprecipitated protein. Insulin and TPA provoked increases in membrane PKC zeta immunoreactivity. The PI 3-kinase inhibitor, wortmannin, suppressed insulin-induced increases in glucose uptake, PI 3-kinase activity, and PKC zeta activation. Wortmannin also suppressed TPA-induced PI 3-kinase activity and PKC zeta activation but suppressed TPA-induced glucose uptake to only a small extent. The PKC inhibitor, Go6976, which only inhibits conventional PKC alpha and -, suppressed TPA-induced glucose uptake, but suppressed insulin-induced glucose uptake to only a small extent. On the other hand, the PKC inhibitor, RO32-0432, which inhibits conventional, novel, and atypical PKCs, markedly suppressed both insulin- and TPA-induced glucose uptake. These results suggest that insulin-induced glucose uptake is mainly mediated by PI 3-kinase-PKC zeta signaling, whereasphorbol ester-induced glucose uptake is mainly mediated by conventional PKC despite PI 3-kinase and PKC zeta activations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:17:40