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Titolo:
Dissection of the humoral immune response toward an immunodominant epitopeof HIV: a model for the analysis of antibody diversity in HIV plus individuals
Autore:
Enshell-Seijffers, D; Smelyanski, L; Vardinon, N; Yust, I; Gershoni, JM;
Indirizzi:
Tel Aviv Univ, George S Wise Fac Life Sci, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 mmunol, IL-69978 Tel Aviv, Israel Tel Aviv Sourasky Med Ctr, Dept Clin Immunol, IL-64239 Tel Aviv, Israel Tel Aviv Sourasky Med Ctr Tel Aviv Israel IL-64239 4239 Tel Aviv, Israel
Titolo Testata:
FASEB JOURNAL
fascicolo: 12, volume: 15, anno: 2001,
pagine: 2112 - 2120
SICI:
0892-6638(200110)15:12<2112:DOTHIR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN MONOCLONAL-ANTIBODIES; RANDOM PEPTIDE LIBRARIES; TRANSMEMBRANE GLYCOPROTEIN; NEUTRALIZING ANTIBODIES; PASSIVE-IMMUNIZATION; PROTECTIVE IMMUNITY; AFFINITY MATURATION; GLOBULIN THERAPY; HUMAN SERA;
Keywords:
AIDS; phage display; humoral response; peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Gershoni, JM Tel Aviv Univ, George S Wise Fac Life Sci, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 978 Tel Aviv, Israel
Citazione:
D. Enshell-Seijffers et al., "Dissection of the humoral immune response toward an immunodominant epitopeof HIV: a model for the analysis of antibody diversity in HIV plus individuals", FASEB J, 15(12), 2001, pp. 2112-2120

Abstract

Understanding the dynamics of the humoral immune response to HIV epitopes in the presence of genetic drift and antigenic variation of the virus may reveal critical elements of protective immunity against HIV. Analysis of antibody maturation and diversity is difficult to study at the molecular levelin humans. We used a combinatorial phage display peptide library to elucidate antibody diversity in HIV-infected individuals to a single immunodominant epitope in gp41. A serum sample derived from an HIV+ individual was usedto screen a phage display a 12 mer cysteine-constrained loop peptide library. In doing so, we isolated mimotope-presenting phages corresponding to the immunodominant gp41 epitope CSGKLIC (residues 603-609). The mimotopes andcontrol phages expressing epitope variants were reacted with a panel of 30HIV+ sera. The patients showed distinct and variable recognition patterns compared with one another. Subfractions of the polyclonal sera were affinity purified and analyzed for epitope specificities. These analyses illustrated that epitope variants can be used to decipher antibody diversity. Elucidation of the plasticity of the humoral response and its polyclonality toward discrete epitopes contributes to our understanding of the antibody maturation process in individuals infected with viruses such as HIV.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:46:58