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Titolo:
MRNA levels of the differentiation-associated linker histone variant H1 zero in mitotically active and postmitotic senescent human diploid fibroblastcell populations
Autore:
Tsapali, DS; Sekeri-Pataryas, KE; Sourlingas, TG;
Indirizzi:
Natl Ctr Sci Res Demokritos, Inst Biol, Athens, Greece Natl Ctr Sci Res Demokritos Athens Greece os, Inst Biol, Athens, Greece
Titolo Testata:
EXPERIMENTAL GERONTOLOGY
fascicolo: 10, volume: 36, anno: 2001,
pagine: 1649 - 1661
SICI:
0531-5565(200111)36:10<1649:MLOTDL>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE ERYTHROLEUKEMIA-CELLS; H1(0) MESSENGER-RNA; IN-VITRO; CORTICAL-NEURONS; CYCLE; EXPRESSION; CHROMATIN; ACCUMULATION; INHIBITORS; H1-DEGREES;
Keywords:
linker histone H1 zero; H1 zero mRNA levels; mitotic HDFs; postmitotic senescent HDFs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Sourlingas, TG Natl Ctr Sci Res Demokritos, Inst Biol, Aghia Paraskevi, Athens, Greece Natl Ctr Sci Res Demokritos Aghia Paraskevi Athens Greece
Citazione:
D.S. Tsapali et al., "MRNA levels of the differentiation-associated linker histone variant H1 zero in mitotically active and postmitotic senescent human diploid fibroblastcell populations", EXP GERONT, 36(10), 2001, pp. 1649-1661

Abstract

The mRNA levels of the linker histone variant Hlo, which is tightly associated with differentiation, have been studied in the present investigation in an in vitro model ageing human diploid fibroblast (HDF) cell system as a function of cumulative population doublings (CPDs) in mitotically active and senescent cell populations. According to our previous findings the synthesis rate of the Hlo protein does not change as a function of CPDs as long as the cells are proliferating. However, when cells reach senescence, the synthesis rate of Hlo increases in both naturally aged as well as in cell populations artificially aged by treatment with sodium butyrate. In the present investigation, it is shown that the Hlo mRNA levels remain relatively constant in mitotic cells with a slight decrease in cell cultures of late CPDs, i.e. in populations which still retain a mitotic potential, but are toward the end of their proliferative Lifespan. However, when cells senesce and are no longer capable of synthesizing DNA, the Hlo mRNA levels increase in naturally aged cells while artificially aged cells still maintain mRNA levels comparable to those of mitotic cells. (C) 2001 Elsevier Science Inc. Allrights reserved.

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Documento generato il 06/04/20 alle ore 05:18:49