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Titolo:
Effects of chloro-s-triazine herbicides and metabolites on aromatase activity in various human cell lines and on vitellogenin production in male carphepatocytes
Autore:
Sanderson, JT; Letcher, RJ; Heneweer, M; Giesy, JP; van den Berg, M;
Indirizzi:
Univ Utrecht, Toxicol Res Inst, Inst Risk Assessment Sci, NL-3508 TD Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TD 3508 TD Utrecht, Netherlands Michigan State Univ, Inst Environm Toxicol, Natl Food Safety & Toxicol Ctr, Dept Zool, E Lansing, MI USA Michigan State Univ E Lansing MI USA l Ctr, Dept Zool, E Lansing, MI USA Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON, Canada Univ Windsor Windsor ON Canada es Inst Environm Res, Windsor, ON, Canada
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 10, volume: 109, anno: 2001,
pagine: 1027 - 1031
SICI:
0091-6765(200110)109:10<1027:EOCHAM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO METABOLISM; BREAST-CANCER; LIVER-MICROSOMES; SPRAGUE-DAWLEY; CYP19 ACTIVITY; WISTAR RATS; ATRAZINE; ASSAY; TERBUTHYLAZINE; TUMORIGENESIS;
Keywords:
antiestrogenic; aromatase; atrazine; carp; chloro-s-triazines; CYP19; estrogenic; H295R; hepatocytes; herbicides; JEG-3; MCF-7; vitellogenin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sanderson, JT Univ Utrecht, Toxicol Res Inst, Inst Risk Assessment Sci, POB 80176, NL-3508 TD Utrecht, Netherlands Univ Utrecht POB 80176 Utrecht Netherlands NL-3508 TD lands
Citazione:
J.T. Sanderson et al., "Effects of chloro-s-triazine herbicides and metabolites on aromatase activity in various human cell lines and on vitellogenin production in male carphepatocytes", ENVIR H PER, 109(10), 2001, pp. 1027-1031

Abstract

We investigated a potential mechanism for the estrogenic properties of three chloro-s-triazine herbicides and six metabolites in vitro in several cell systems. We determined effects on human aromatase (CYP19), the enzyme that converts androgens to estrogens, in H295R (adrenocortical carcinoma), JEG-3 (placental choriocarcinoma), and MCF-7 (breast cancer) cells; we determined effects on estrogen receptor-mediated induction of vitellogenin in primary hepatocyte cultures of adult male carp (Cyprinus carpio). In addition to atrazine, simazine, and propazine, two metabolites-atrazine-desethyl and atrazine-desisopropyl-induced aromatase activity in H295R cells concentration-dependently (0.3-30 muM) and with potencies similar to those of the parent triazines. After a 24-hr exposure to 30 muM of the triazines, an apparent maximum induction of about 2- to 2.5-fold was achieved. The induction responses were confirmed by similar increases in CYP19 mRNA levels, determinedby reverse-transcriptase polymerase chain reaction. In JEG-3 cells, where basal aromatase expression is about 15-fold greater than in H295R cells, the induction responses were similar but less pronounced; aromatase expression in MCF-7 cells was neither detectable nor inducible under our culture conditions. The fully dealkylated metabolite atrazine-desethyl-desisopropyl and the three hydroxylated metabolites (2-OH-atrazine-desethyl, -desisopropyl, and -desethyl-desisopropyl) did not induce aromatase activity, None of the triazine herbicides nor their metabolites induced vitellogenin productionin male carp hepatocytes; nor did they antagonize the induction of vitellogenin by 100 nM (EC50) 17 beta -estradiol. These findings together with other reports indicate that the estrogenic effects associated with the triazine herbicides in vivo are not estrogen receptor-mediated, but may be explained partly by their ability to induce aromatase in vitro.

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Documento generato il 18/01/20 alle ore 07:34:26