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Titolo:
Microfluorimetric analysis of a purinergic receptor (P2X(7)) in GH(4)C(1) rat pituitary cells: Effects of a bioactive substance produced by Pfiesteria piscicida
Autore:
Melo, AC; Moeller, PDR; Glasgow, H; Burkholder, JM; Ramsdell, JS;
Indirizzi:
NOAA, Coastal Res Branch, Ctr Coastal Environm Hlth & Biomol Res, Natl Ocean Serv,Marine Biotoxins Program, Charleston, SC 29412 USA NOAA CharlestonSC USA 29412 Biotoxins Program, Charleston, SC 29412 USA N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 ept Bot, Raleigh, NC 27695 USA
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
, volume: 109, anno: 2001, supplemento:, 5
pagine: 731 - 737
SICI:
0091-6765(200110)109:<731:MAOAPR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHARMACOLOGICAL CHARACTERIZATION; MICROGLIAL CELLS; CATION CHANNELS; P-2Z RECEPTOR; ATP; DINOFLAGELLATE; ACTIVATION; PURINORECEPTOR; MAITOTOXIN; EXPRESSION;
Keywords:
GH(4)C(1); Pfiesteria; purinergic receptors; P2X(7); toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Ramsdell, JS NOAA, Coastal Res Branch, Ctr Coastal Environm Hlth & Biomol Res, Natl Ocean Serv,Marine Biotoxins Program, 219 Ft Johnson Rd, Charleston, SC 29412 USA NOAA 219 Ft Johnson Rd Charleston SC USA 29412 , SC 29412 USA
Citazione:
A.C. Melo et al., "Microfluorimetric analysis of a purinergic receptor (P2X(7)) in GH(4)C(1) rat pituitary cells: Effects of a bioactive substance produced by Pfiesteria piscicida", ENVIR H PER, 109, 2001, pp. 731-737

Abstract

Pfiesteria piscicida Steidinger & Burkholder is a toxic dinoflagellate that leads to fish and human toxicity. It produces a bioactive substance that leads to cytotoxicity of GH(4)C(1) rat pituitary cells. Extracellular adenosine F-triphosphate (ATP) acting on P2X(7) purinergic receptors induces theformation of a nonselective cation channel, causing elevation of the cytosolic free calcium followed by a characteristic permeabilization of the cellto progressively larger ions and subsequent cell lysis. We investigated whether GH4C1 rat pituitary cells express functional P2X7 receptors, and if so, are they activated by a bioactive substance isolated from toxic P. piscicida cultures. We tested the selective agonist 2'-3'-O-(benzoyl-4-benzoyl)-ATP (BzATP) and antagonists piridoxalphosphate-6-azophenyl-2'-4'-disulfonicacid (PPADS) and oxidized-ATP (oxATP) using elevated cytosolic free calcium in Fura-2 loaded cells, and induced permeability of these cells to the fluorescent dye YO-PRO-1 as end points. We demonstrated that in GH(4)C(1) cells, BzATP induces both the elevation of cytosolic free calcium and the permeabilization of the cell membrane. ATP-induced membrane permeabilization was inhibited by PPADS reversibly and by oxATP irreversibly. The putative Pfiesteria toxin (pPfTx) also elevated cytosolic free calcium in Fura-2 in GH(4)C(1) cells and increased the permeability to YO-PRO-1 in a manner inhibited fully by oxATP. This study indicates that GH(4)C(1) cells express a purinoceptor with characteristics consistent with the P2X7 subtype, and that pPfTx mimics the kinetics of cell permeabilization by ATP.

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Documento generato il 28/11/20 alle ore 09:03:23