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Titolo:
Identification and characterization of two distinct GnRH receptor subtypesin a teleost, the medaka Oryzias latipes
Autore:
Okubo, K; Nagata, S; Ko, R; Kataoka, H; Yoshiura, Y; Mitani, H; Kondo, M; Naruse, K; Shima, A; Aida, K;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 , Dept Aquat Biosci, Tokyo 1138657, Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 t Integrated Biosci, Tokyo 1138657, Japan Univ Tokyo, Grad Sch Frontier Sci, Tokyo 1130033, Japan Univ Tokyo TokyoJapan 1130033 ad Sch Frontier Sci, Tokyo 1130033, Japan Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sci, Dept Biol Sci, Tokyo 1130033, Japan
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 11, volume: 142, anno: 2001,
pagine: 4729 - 4739
SICI:
0013-7227(200111)142:11<4729:IACOTD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; VERTEBRATE GENOME EVOLUTION; GOLDFISH CARASSIUS-AURATUS; C-TERMINAL TAIL; INTERNALIZATION KINETICS; DEVELOPMENTAL GENETICS; TISSUE DISTRIBUTION; LIGAND SELECTIVITY; MOLECULAR-CLONING; RAINBOW-TROUT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Aida, K Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 quat Biosci, Tokyo 1138657, Japan
Citazione:
K. Okubo et al., "Identification and characterization of two distinct GnRH receptor subtypesin a teleost, the medaka Oryzias latipes", ENDOCRINOL, 142(11), 2001, pp. 4729-4739

Abstract

We report the identification and characterization of two distinct GnRH receptor (GnRH-R) subtypes, designated GnRH-R1 and GnRH-R2, in a model teleost, the medaka Ory. zias latipes. These seven-transmembrane receptors of the medaka contain a cytoplasmic C-terminal tail, which has been found in all other nonmammalian GnRH-Rs cloned to date. The GnRH-R1 gene is composed of three exons separated by two introns, whereas the GnRH-R2 gene has an additional intron and therefore consists of four exons and three introns. The GnRH-R1 and GnRH-R2 genes, both of which exist as single-copy genes in the medaka genome, were mapped to linkage groups 3 and 16, respectively. Inositol phosphate assays using COS-7 cells transfected with GnRH-R1 and GnRH-R2 demonstrated that they had remarkably different ligand sensitivities, althoughboth receptors showed highest preference for chicken-II-type GnRH. Phylogenetic analysis showed the presence of three paralogous lineages for vertebrate GnRH-Rs and indicated that neither GnRH-R1 nor GnRH-R2 is the medaka ortholog to mammalian GnRH-Rs that lack a cytoplasmic tail. This, together with an observation that medaka-type GnRH had low affinity for GnRH-R1 and GnRH-R2, suggests that a third GnRH-R may exist in the medaka.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:51:16