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Titolo:
Rapid analysis of amino sugars by microchip electrophoresis with laser-induced fluorescence detection
Autore:
Suzuki, S; Shimotsu, N; Honda, S; Arai, A; Nakanishi, H;
Indirizzi:
Kinki Univ, Fac Pharmaceut Sci, Higashiosaka, Osaka 577, Japan Kinki UnivHigashiosaka Osaka Japan 577 i, Higashiosaka, Osaka 577, Japan Shimadzu Co Ltd, Technol Res Lab, Kyoto, Japan Shimadzu Co Ltd Kyoto Japan madzu Co Ltd, Technol Res Lab, Kyoto, Japan
Titolo Testata:
ELECTROPHORESIS
fascicolo: 18, volume: 22, anno: 2001,
pagine: 4023 - 4031
SICI:
0173-0835(200110)22:18<4023:RAOASB>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY ZONE ELECTROPHORESIS; REDUCING MONOSACCHARIDES; SAMPLE INJECTION; BORATE COMPLEXES; DERIVATIZATION; DERIVATIVES; ACIDS; 4-FLUORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE; OLIGOSACCHARIDES;
Keywords:
microchip electrophoresis; 1-min analysis; 4-nitro-2,1,3-benzoxadiazole; NBD-amino sugars; hexosamines; hexosaminitols; O-linked glycoprotein glycans; bovine submaxillary mucin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Honda, S Kinki Univ, Fac Pharmaceut Sci, 3-4-1 Kowakae, Higashiosaka, Osaka 577, Japan Kinki Univ 3-4-1 Kowakae Higashiosaka Osaka Japan 577 577, Japan
Citazione:
S. Suzuki et al., "Rapid analysis of amino sugars by microchip electrophoresis with laser-induced fluorescence detection", ELECTROPHOR, 22(18), 2001, pp. 4023-4031

Abstract

Microchip electrophoresis for the short-time analysis of amino sugars is described. D-Glucosamine, D-galactosamine and their reduced forms were labeled with 4-nitro-, 2,1,3-benzoxadiazole 7-fluoride (NBD-F) at pH 6.0 and thefluorescent derivatives were purified on an octadecyl silica (ODS) gel plate. The derivatives were analyzed by electrophoresis on a microfabricated chip with a 33 mm long separation channel with Corporation, argon laser-induced fluorescence detection. Under the established conditions, these amino sugar derivatives were well separated from each other within 60 s. Amino sugars of as small an amount as 0.5 fmol could be detected with a signal-to-noise (S/N) ratio of 3, and peak response showed good linearity between at least 0.8 and 8 fmol of samples with a relative standard deviation (RSD) of ca. 4%. This method was also applied to the analysis of amino sugar composition of O-linked glycans released from bovine submaxillary mucin with alkaliin the presence of borohydride. The result of amino sugar composition analysis for individual O-glycans fractionated by high-performance liquid chromatography was quite useful for their identification.

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Documento generato il 24/10/20 alle ore 20:44:55