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Titolo:
Site-specific regulation of oestrogen receptor-alpha and -beta by oestradiol in human adipose tissue
Autore:
Anwar, A; McTernan, PG; Anderson, LA; Askaa, J; Moody, CG; Barnett, AH; Eggo, MC; Kumar, S;
Indirizzi:
Univ Birmingham, Queen Elizabeth Hosp, Dept Med, Birmingham B15 2TH, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TH W Midlands, England Birmingham Heartlands Hosp, Birmingham B9 5ST, W Midlands, England Birmingham Heartlands Hosp Birmingham W Midlands England B9 5ST , England DAKO AS, DK-2600 Glostrup, Denmark DAKO AS Glostrup Denmark DK-2600DAKO AS, DK-2600 Glostrup, Denmark
Titolo Testata:
DIABETES OBESITY & METABOLISM
fascicolo: 5, volume: 3, anno: 2001,
pagine: 338 - 349
SICI:
1462-8902(200110)3:5<338:SROORA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN RESPONSE ELEMENT; LIPOPROTEIN-LIPASE; GENDER DIFFERENCES; IN-VITRO; ER-BETA; IDENTIFICATION; ADIPOCYTES; BINDING; HETERODIMERS; RESISTANCE;
Keywords:
oestrogen receptors; human adipose tissue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kumar, S Univ Birmingham, Queen Elizabeth Hosp, Dept Med, Clin Res Block, Birmingham B15 2TH, W Midlands, England Univ Birmingham Clin Res Block Birmingham W Midlands England B15 2TH
Citazione:
A. Anwar et al., "Site-specific regulation of oestrogen receptor-alpha and -beta by oestradiol in human adipose tissue", DIABET OB M, 3(5), 2001, pp. 338-349

Abstract

Aim To examine the expression of oestrogen receptors alpha and alpha (ER alpha and ER beta) and their regulation by 17 beta -oestradiol (E-2) in stromal cells and adipocytes from human subcutaneous (s.c.) and omental (o.m.) adipose tissue. Methods Subcutaneous and o.m. abdominal adipose tissues were obtained from10 women (mean age 63.5 +/-4.8 years; mean weight 75.6 +/-6.7 kg) undergoing elective or cosmetic surgery. Immunohistochemistry and RT-PCR analysis were used to detect the presence of ER alpha and ER beta. The regulation of ER alpha and ER beta by E-2 (10(-7) M to 10-(9) M) was examined using Western immunoblotting analysis in both s.c. and o.m. stromal cells and mature adipocytes cultured in serum-free, phenol red-free medium. Results Immunostaining of s.c. and o.m. adipose tissue showed that the ER subtypes were localized predominantly within the nucleus. Western analysis demonstrated that E-2 treatments differentially altered ER alpha and ER beta expression in s.c. and o.m. adipocytes. In s.c. and o.m. stromal cells, E-2 (10(-8) M) produced a significant up regulation relative to control of 66 kDa ERa (s.c.:1.87 +/-0.22; o.m.:1.97 +/-0.17; p<0.05) and 60 kDa ER<beta> (s.c.:1.66 +/-0.3; o.m.: 1.68 +/-0.16; p<0.05). In s.c. adipocytes, however, ER<alpha> expression significantly decreased with E-2 10(-8) M relativeto control while ER beta expression increased (ER alpha 0.58 +/-0.06, ER beta: 1.47 +/-0.11; p<0.05). In o.m. adipocytes, the inhibition of ER<alpha>with E-2 was not observed (ER alpha 1.86 +/-0.36, ER beta :1.03 +/-0.15, p<0.05)Conclusions ER<alpha> and ER beta are expressed but differentially regulated by E2 in s.c. and o.m. adipocytes and stromal cells. The upregulation ofER beta by E-2 suggests that E-2 maintains the expression of these receptors. The feed-back inhibition of ER alpha expression by E-2 in s.c. but not o.m. adipocytes observed in vitro is consistent with the data from ER alphaknock out mice where s.c. fat is increased. Selective ER modulators may have different effects in different adipose sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:53:33