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Titolo:
Lipoprotein lipase gene polymorphism and lipid profile in patients with hypertriglyceridemia
Autore:
Pasalic, D; Sertic, J; Kunovic, B; Milicevic, Z; Pasic, A; Zrinski-Topic, R; Ferencak, G; Stavljenic-Rukavina, A;
Indirizzi:
Univ Zagreb, Sch Med, Dept Chem & Biochem, Zagreb 10000, Croatia Univ Zagreb Zagreb Croatia 10000 t Chem & Biochem, Zagreb 10000, Croatia Univ Zagreb, Ctr Hosp, Clin Inst Lab Diag, Zagreb 41000, Croatia Univ Zagreb Zagreb Croatia 41000 in Inst Lab Diag, Zagreb 41000, Croatia Zagreb Hlth Ctr, Cent Diagnost Lab, Zagreb, Croatia Zagreb Hlth Ctr Zagreb Croatia Ctr, Cent Diagnost Lab, Zagreb, Croatia Univ Clin Diabet Endocrinol & Metab Dis, Vuk Vrhovac Inst, Dept Metab Disorders, Zagreb, Croatia Univ Clin Diabet Endocrinol & Metab Dis Zagreb Croatia Zagreb, Croatia Univ Zagreb, Ctr Hosp, Dept Dermatol & Venereol, Zagreb 41000, Croatia Univ Zagreb Zagreb Croatia 41000 matol & Venereol, Zagreb 41000, Croatia
Titolo Testata:
CROATIAN MEDICAL JOURNAL
fascicolo: 5, volume: 42, anno: 2001,
pagine: 517 - 522
SICI:
0353-9504(200110)42:5<517:LLGPAL>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DENSITY-LIPOPROTEIN; DNA POLYMORPHISMS; MISSENSE MUTATION; LPL GENE; TRIGLYCERIDE LEVELS; ASSOCIATIONS; DEFICIENCY; DISEASE; METABOLISM; SEVERITY;
Keywords:
apolipoprotein A-I; apolipoproteins B; Croatia; lipoproteins, HDL cholesterol; lipoprotein lipase; polymorphism, restriction fragment length; triglycerides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Pasalic, D Univ Zagreb, Sch Med, Dept Chem & Biochem, Salata 3, Zagreb 10000, Croatia Univ Zagreb Salata 3 Zagreb Croatia 10000 agreb 10000, Croatia
Citazione:
D. Pasalic et al., "Lipoprotein lipase gene polymorphism and lipid profile in patients with hypertriglyceridemia", CROAT MED J, 42(5), 2001, pp. 517-522

Abstract

Aim. To assess lipid profile and the genotype distribution of lipoprotein lipase gene polymorphism at Pvu II polymorphic site within the intron between exons 6 and 7 in patients with hypertriglyceridemia. Methods. Pvu II polymorphism was determined in 116 hypertriglyceridemic patients and 50 normolipidemic controls from Zagreb, Croatia. DNA was extracted from peripheral blood mononuclear cells. Polymerase chain reaction was used for amplification of 6th intron, which was then restricted with Pvu II-restriction endonuclease. Serum lipid and lipoprotein fractions were determined by standard enzymatic methods. Cholesterol concentrations in HDL subfractions, HDL2 and HDL3, were determined after precipitation with polyethyleneglycol. Apolipoproteins (apo) A-I and B were determined by immunonephelometry. Results. Triglycerides showed a positive correlation with total cholesterol (r=0.222, 95% Cl = 0.041-0.389, p=0.017) and inverse correlation with HDL-cholesterol (r=-0.278, 95% Cl=-0.449 to -0.088, p=0.005), especially with HDL3-cholesterol (r=-0.333, 95% Cl=-0.497 to -0.147, p=0.001). The respective frequencies for genotypes -/-, +/-,and +/+ were 22, 58,and 36 in the patient group, and 17,17,and 16 in the control group. Serum triglycerides in the patient group, expressed as median in mmol/L, were 3.30 (range, 2.60-10.90), 3.60 (range, 2.50-21.50), and 3.99 (range, 2.50-15.56), respectively. Serum concentration of triglycerides differed significantly between the +/and genotype (p = 0.043). Conclusion. There is an association between genetic variation at the locusfor lipoprotein lipase and high serum triglyceride levels. This might prove useful in the detection of individuals susceptible to the development of hypertriglyceridemia, as well as a marker in the analysis of this genetic defect in patient families.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 22:54:46