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Titolo:
Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608
Autore:
Sanchez, M; Gella, FJ; Profilis, C; Ceriotti, F; Cuso, E; Fuentes-Arderiu, X; Hallermayer, K; Horder, M; Panteghini, M; Schumann, G; Canalias, F;
Indirizzi:
Univ Autonoma Barcelona, Unitat Bioquim Med, Dept Bioquim & Biol Mol, E-08193 Bellaterra, Spain Univ Autonoma Barcelona Bellaterra Spain E-08193 08193 Bellaterra, Spain Commiss European Communities, B-1049 Brussels, Belgium Commiss European Communities Brussels Belgium B-1049 9 Brussels, Belgium San Raffaele Sci Inst, Lab Anal, I-20132 Milan, Italy San Raffaele Sci Inst Milan Italy I-20132 Lab Anal, I-20132 Milan, Italy Balague Ctr SA, Lhospitalet De Llobregat, Spain Balague Ctr SA Lhospitalet De Llobregat Spain talet De Llobregat, Spain Ciudad Sanitaria & Univ Bellvitge, Lhospitalet De Llobregat, Spain Ciudad Sanitaria & Univ Bellvitge Lhospitalet De Llobregat Spain Spain Roche Diagnost GmbH, Penzberg, Germany Roche Diagnost GmbH Penzberg Germany e Diagnost GmbH, Penzberg, Germany Odense Univ Hosp, KKA, Dept Clin Biochem & Clin Genet, DK-5000 Odense, Denmark Odense Univ Hosp Odense Denmark DK-5000 n Genet, DK-5000 Odense, Denmark Spedali Civil Brescia, Azienda Osped, Clin Chem Lab 1, I-25125 Brescia, Italy Spedali Civil Brescia Brescia Italy I-25125 ab 1, I-25125 Brescia, Italy Inst Klin Chem, Hannover, Germany Inst Klin Chem Hannover GermanyInst Klin Chem, Hannover, Germany
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 9, volume: 39, anno: 2001,
pagine: 858 - 865
SICI:
1434-6621(200109)39:9<858:COTMCO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATALYTIC CONCENTRATION; N-PHOSPHOTRANSFERASE; BIOCHEMICAL MARKERS; IFCC METHODS; STANDARDIZATION; RECOMMENDATION; COMMITTEE; PROTEIN; ENZYMES; EC-2732;
Keywords:
creatine kinase-2; reference material; standardization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Canalias, F Univ Autonoma Barcelona, Unitat Bioquim Med, Dept Bioquim & Biol Mol, EdifM, E-08193 Bellaterra, Spain Univ Autonoma Barcelona Edif M Bellaterra Spain E-08193 Spain
Citazione:
M. Sanchez et al., "Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608", CLIN CH L M, 39(9), 2001, pp. 858-865

Abstract

We describe the certification of a mass concentration value in the alreadyprepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2was purified from human heart. The purified enzyme was diluted in order tomeasure its protein concentration by the Doetsch method. A protein concentration value of 124.30 +/- 13.17 mg/l was assigned to the stock solution ofpurified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 mug/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 materialby immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30 +/- 9.65 mug/l.

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Documento generato il 04/04/20 alle ore 08:53:37