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Titolo:
Detection and quantification of small numbers of circulating tumour cells in peripheral blood using laser scanning cytometer (LSC (R))
Autore:
Pachmann, K; Heiss, P; Demel, U; Tilz, G;
Indirizzi:
LKH Univ Graz, Jean Dausset Lab, Gemeinsame Einrichtung Klin Immunol, Graz, Austria LKH Univ Graz Graz Austria same Einrichtung Klin Immunol, Graz, Austria Lab Spezielle Immunhamatol & Gendiagnost, Bayreuth, Germany Lab Spezielle Immunhamatol & Gendiagnost Bayreuth Germany euth, Germany
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 9, volume: 39, anno: 2001,
pagine: 811 - 817
SICI:
1434-6621(200109)39:9<811:DAQOSN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER CELLS; POLYMERASE-CHAIN-REACTION; MINIMAL RESIDUAL DISEASE; HIGH-DOSE CHEMOTHERAPY; IMMUNOCYTOCHEMICAL DETECTION; CARCINOMA CELLS; BONE-MARROW; RT-PCR; CYTOKERATIN-19;
Keywords:
solid tumours; minimal residual disease; laser scanning cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Pachmann, K Univ Jena, Klin Innere Med 2, Erlanger Allee 101, D-07740 Jena, Germany Univ Jena Erlanger Allee 101 Jena Germany D-07740 na, Germany
Citazione:
K. Pachmann et al., "Detection and quantification of small numbers of circulating tumour cells in peripheral blood using laser scanning cytometer (LSC (R))", CLIN CH L M, 39(9), 2001, pp. 811-817

Abstract

The detection of circulating tumour cells disseminated from solid tumours requires extremely sensitive methods. Molecular genetic methods, which are most sensitive, are not applicable to solid tumours because no tumour-specific genetic markers are available. Detection of disseminated tumour cells by immunocytochemistry is time-consuming, whereas fluorimetry is fast and quantitative. The laser scanning cytometer (LSC (R)) provides an automated microscopic procedure for screening Up to 5 x 10(4) cells in suitable time. Using this system together with an enrichment procedure which allows up to ten thousand-fold enrichment, we have quantified minimal numbers of tumour cells. In a model system, breast cancer cell line cells diluted into peripheral blood mimicked seeding of tumour cells into the periphery. After staining with fluorochrome-conjugated anti-epithelial antibody, slides were screened for positive events directly or after enrichment with antibody-coated magnetic beads. One positive cell was unequivocally detectable in 104 cells and 50 out of 60 tumour cells were reliably recovered from a 20 ml blood volume, equal to 1-2 cells per 10(7), after magnetic bead enrichment. This method allows quantitation of tumour cells in peripheral blood and bone marrow in reasonable time and will, for the first time, enable extensive investigation of the seeding behaviour of tumours.

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Documento generato il 13/07/20 alle ore 03:27:58