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Titolo:
Routine differential diagnosis of proteinurias by capillary electrophoresis
Autore:
Kolios, G; Bairaktari, E; Tsolas, O; Seferiadis, K;
Indirizzi:
Univ Ioannina, Sch Med, Biochem Lab, Reg Univ Gen Hosp Ioannina, Ioannina 45500, Greece Univ Ioannina Ioannina Greece 45500 osp Ioannina, Ioannina 45500, Greece
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 9, volume: 39, anno: 2001,
pagine: 784 - 788
SICI:
1434-6621(200109)39:9<784:RDDOPB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOGLOBIN-VARIANTS; URINARY PROTEIN; MICROALBUMINURIA;
Keywords:
capillary electrophoresis; proteinuria; tubular proteinuria; glomerular proteinuria; Bence-Jones protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Seferiadis, K Univ Ioannina, Sch Med, Biochem Lab, Reg Univ Gen Hosp Ioannina, Ioannina 45500, Greece Univ Ioannina Ioannina Greece 45500 Ioannina 45500, Greece
Citazione:
G. Kolios et al., "Routine differential diagnosis of proteinurias by capillary electrophoresis", CLIN CH L M, 39(9), 2001, pp. 784-788

Abstract

Capillary electrophoresis is a relatively new analytical technique that begins to have an impact on both routine and research in clinical laboratories. Recently, a fully automated system has become commercially available (Paragon CZE 2000, Beckman, USA) for the analysis of human serum proteins. Urine protein analysis, on the other hand, is currently accomplished by electrophoresis of concentrated urine specimens. The method is used to distinguish the glomerular from the tubular proteinuria and for the identification ofBence-Jones proteins. The procedure is labor-intensive and technically demanding. We developed a technique for the serum capillary electrophoresis instrument that can also be applied routinely to the differential diagnosis of proteinurias. Overriding the programmed dilution step of the instrument, we were able to distinguish different types of proteinurias without concentration of specimens with a total protein content of 150-200 mg/l as determined by sulfosalicylic acid. The different electrophoretic patterns obtainedby the capillary electrophoresis system for various specimens correlated well with established techniques (Hydragel Proteinurie Kit, Sebia, France). The method is applicable for routine analysis of urinary proteins. It is reliable, less expensive and faster than the conventional methods (electrophoretic or immunonephelometric) used today for the differentiation of proteinurias, and it can be used as a quick screening test.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:53:47