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Titolo:
A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases
Autore:
Ikemoto, M; Tsunekawa, S; Awane, M; Fukuda, Y; Murayama, H; Igarashi, M; Nagata, A; Kasai, Y; Totani, M;
Indirizzi:
Kyoto Univ, Coll Med Technol, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 v, Coll Med Technol, Kyoto 6068507, Japan NTT W Kyoto Hosp, Dept Surg, Kyoto 6018441, Japan NTT W Kyoto Hosp KyotoJapan 6018441 sp, Dept Surg, Kyoto 6018441, Japan Kyoto Univ, Grad Sch Med, Dept Surg Gastroenterol, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Surg Gastroenterol, Kyoto 6068507, Japan Yamasa Shoyu Co Ltd, Div Diagnost, Immunol Lab, Chiba 2880056, Japan Yamasa Shoyu Co Ltd Chiba Japan 2880056 mmunol Lab, Chiba 2880056, Japan Kyoto Univ Hosp, Cent Clin Lab, Kyoto 6068507, Japan Kyoto Univ Hosp Kyoto Japan 6068507 Cent Clin Lab, Kyoto 6068507, Japan Natl Inst Hlth & Nutr, Shinjuku Ku, Tokyo 1628636, Japan Natl Inst Hlth & Nutr Tokyo Japan 1628636 njuku Ku, Tokyo 1628636, Japan
Titolo Testata:
CLINICAL BIOCHEMISTRY
fascicolo: 6, volume: 34, anno: 2001,
pagine: 455 - 461
SICI:
0009-9120(200109)34:6<455:AUESFH>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBODY; PURIFICATION; HEPATECTOMY;
Keywords:
liver-type arginase; ELISA; liver injury; hemoglobin; monoclonal antibody; liver disease; urea cycle; erythrocytes; kidney;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ikemoto, M Kyoto Univ, Coll Med Technol, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Technol, Kyoto 6068507, Japan
Citazione:
M. Ikemoto et al., "A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases", CLIN BIOCH, 34(6), 2001, pp. 455-461

Abstract

Objectives: To develop a new ELISA system for liver-type arginase using monoclonal antibodies against the enzyme, and to verify the utility of the arginase in diagnosis of hepatic disorders. Design and Methods: We have developed an enzyme-linked immunosorbent assay(ELISA), using two kinds of monoclonal antibodies (Mo6G3 and Mo9C5) for human liver-type arginase as the first and second antibodies respectively. Wehave also developed a new method to eliminate the influence of erythrocyte-derived arginase contamination in hemolytic samples. This ELISA was applied to specimens received from patients with acute and chronic hepatic disease and also patients who had undergone partial hepatectomy. Results: This assay is sensitive and reproducible for the measurement of liver-type arginase in the sera of patients with liver dysfunction, and enabled us to detect enzyme concentrations as low as 27 pmol/L without any processing of the samples. The assay showed with-in-run coefficients of variation (CV) ranging from 1.9 to 4.1% and between-day CV from 3.6 to 5.1% for arginase concentrations varing from 57.1 to 1200 pmol/L. The recovery was 113% (mean) with a range of 96 to 129%. These antibodies reacted strongly withboth recombinant and native liver-type arginases, while, to some extent, with erythrocyte-derived arginase. Correction for erythrocyte-derived arginase contamination in hemolytic samples was, however, easily made by assayingperoxidase-like activity of hemoglobin. From the view of a limited localization of arginase in the liver, the marked increase of the enzyme in serum reflects initiation of liver injury, while the rapid decrease reflects termination of the damage. Such quick normalization in circulating liver-type arginase indicated another merit of the enzyme in diagnosis of liver diseases. Conclusions: The changes in circulating liver-type arginase level could behelpful not only in the diagnosis of liver diseases but also subsequent treatment of the patients with liver damage. (C) 2001 The Canadian Society ofClinical Chemists. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 14:12:24