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Titolo:
Examination of oncogene amplification by genomic DNA microarray in hepatocellular carcinomas: comparison with comparative genomic hybridization analysis
Autore:
Takeo, S; Arai, H; Kusano, N; Harada, T; Furuya, T; Kawauchi, S; Oga, A; Hirano, T; Yoshida, T; Okita, K; Sasaki, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Pathol, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Internal Med, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 305, Japan Natl Inst Biosci & Human Technol Tsukuba Ibaraki Japan 305 aki 305, Japan
Titolo Testata:
CANCER GENETICS AND CYTOGENETICS
fascicolo: 2, volume: 130, anno: 2001,
pagine: 127 - 132
SICI:
0165-4608(20011015)130:2<127:EOOABG>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
COPY NUMBER CHANGES; GENE AMPLIFICATION; HUMAN NEOPLASMS; EXPRESSION; PROGNOSIS; TUMORS; TOOL; EMS1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Takeo, S Yamaguchi Univ, Sch Med, Dept Pathol, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 aguchi 7558505, Japan
Citazione:
S. Takeo et al., "Examination of oncogene amplification by genomic DNA microarray in hepatocellular carcinomas: comparison with comparative genomic hybridization analysis", CANC GENET, 130(2), 2001, pp. 127-132

Abstract

To identify amplified oncogenes involved in hepatocellular carcinomas (HCC), we applied a genomic DNA microarray spotted with 57 oncogenes to 20 HCCs. Aberrations in DNA copy number also were analyzed by comparative genomic hybridization (CGH) using an aliquot of DNA samples. In 5 of 20 HCCs, only 6 oncogenes (CCND1, FGF3/FGF4, SAS/CDK4, TERC, MET, and MYC) were amplified, whereas in the remaining 15 tumors no oncogenes were amplified. A comparison of DNA microarray and conventional CGH analyses showed that, although 5of 6 amplified oncogenes shown by microarray were located in chromosomal regions shown by CGH to have increased DNA copy numbers, not all genes located in such chromosomal regions were affected. One of the amplified oncogenes (SAS/CDK4) was found in a chromosomal region that was undetected by CGH. We, therefore, conclude that amplification of the oncogenes examined in this series is not directly implicated in hepatocellular carcinogenesis. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:25:52