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Titolo:
Extracellular production of biologically active deacetoxycephalosporin C synthase from Streptomyces clavuligerus in Pichia pastoris
Autore:
Adrio, JL; Velasco, J; Soler, G; Rodriguez-Saiz, M; Barredo, JL; Moreno, MA;
Indirizzi:
SAU, Antibiot, Dept I&D, Lab Bioquim, Leon 24009, Spain SAU Leon Spain 24009 Antibiot, Dept I&D, Lab Bioquim, Leon 24009, Spain SAU, Antibiot, Dept I&D, Lab Biotecnol, Leon 24009, Spain SAU Leon Spain24009 ntibiot, Dept I&D, Lab Biotecnol, Leon 24009, Spain
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 4, volume: 75, anno: 2001,
pagine: 485 - 491
SICI:
0006-3592(20011120)75:4<485:EPOBAD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PENICILLIN-G; CEPHALOSPORIN BIOSYNTHESIS; METHYLOTROPHIC YEAST; ESCHERICHIA-COLI; GENE-EXPRESSION; ACREMONIUM; STRAINS; ENZYME; IMMOBILIZATION; STABILIZATION;
Keywords:
deacetoxycephalosporin C synthase; expandase; Pichia pastoris; penicillin; cephalosporin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Moreno, MA SAU, Antibiot, Dept I&D, Lab Bioquim, Avda Antibiot 59-61, Leon24009, Spain SAU Avda Antibiot 59-61 Leon Spain 24009 61, Leon 24009, Spain
Citazione:
J.L. Adrio et al., "Extracellular production of biologically active deacetoxycephalosporin C synthase from Streptomyces clavuligerus in Pichia pastoris", BIOTECH BIO, 75(4), 2001, pp. 485-491

Abstract

We have successfully expressed and observed secretion of the Streptomyces clavuligerus deacetoxycephalosporin C synthase (DAOCS) using the Pichia pastoris expression system. Two clones having multiple copies of the expression cassette were selected and used for protein-expression analysis. SDS-PAGEshowed efficient expression and secretion of the bacteria[ recombinant DAOCS. The highest yield (120 mug/mL) was obtained when expression was inducedwith 2% methanol. Free and immobilized protein were assayed for biologicalactivity and found to expand penicillin N (its natural substrate) and penicillin G to deacetoxycephalosporin C (DAOC) and deacetoxycephalosporin G (DAOG), respectively. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:17:22