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Titolo:
Thermodynamic basis for the increased thermostability of CheY from the hyperthermophile Thermotoga maritima
Autore:
Deutschman, WA; Dahlquist, FW;
Indirizzi:
Univ Oregon, Dept Chem, Inst Mol Biol, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 Chem, Inst Mol Biol, Eugene, OR 97403 USA
Titolo Testata:
BIOCHEMISTRY
fascicolo: 43, volume: 40, anno: 2001,
pagine: 13107 - 13113
SICI:
0006-2960(20011030)40:43<13107:TBFTIT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-RESIDUES; HEAT-CAPACITY; ENHANCED THERMOSTABILITY; CRYSTAL-STRUCTURES; THERMAL-STABILITY; PROTEINS; DEHYDROGENASE; NETWORKS; ENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Dahlquist, FW Univ Oregon, Dept Chem, Inst Mol Biol, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 Biol, Eugene, OR 97403 USA
Citazione:
W.A. Deutschman e F.W. Dahlquist, "Thermodynamic basis for the increased thermostability of CheY from the hyperthermophile Thermotoga maritima", BIOCHEM, 40(43), 2001, pp. 13107-13113

Abstract

The CheY protein isolated from the hyperthermophile Thermotoga maritima ismuch more resistant to thermally induced unfolding than is its counterpartfrom the mesophile Bacillus subtilis. To determine the basis of this increased thermostability, the temperature dependence of the free energy of unfolding was determined for these CheY homologues using denaturant-induced unfolding experiments. This allowed comparison of T. maritima CheY with B. subtilis CheY and determination of the thermodynamic qualities responsible forthe enhanced thermostability of T. maritima CheY. The stability of the thermophilic CheY protein is a direct result of the increased enthalpy contribution at the temperature of zero entropy, T-s, and the decreased heat capacity change upon unfolding, resulting in a decreased dependence of the free energy of unfolding on temperature. It was found that neither purely entropic nor purely enthalpic contributions alone (as reflected by T (s)) were sufficient to account for the increase in stability.

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Documento generato il 10/07/20 alle ore 15:00:30